Carbon nanotube thin film biosensors for sensitive and reproducible whole virus detection.

Carbon nanotube thin film biosensors for sensitive and reproducible whole virus detection.
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碳纳米管薄膜生物传感器,用于敏感和可重复的整个病毒检测。

DOI:
10.7150/thno.3726
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发表时间:
2012
期刊:
影响因子:
12.4
通讯作者:
Tang XS
Tang XS
中科院分区:
医学1区
文献类型:
--
作者:
Mandal HS;Su Z;Ward A;Tang XS

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在这里,我们报告了使用碳纳米管薄膜(CNT-TF)场效应器件对整个病毒进行无标记、灵敏和实时的电检测。使用简单的两端(无门电极)配置演示了对大约 550 种模型病毒(M13 噬菌体)的选择性检测。通过特异性抗体-病毒结合在碳纳米管表面的化学门控被认为是传感机制。与具有相同微电极尺寸(无 CNT)的电阻抗传感器相比,CNT-TF 传感器的灵敏度高出 5 个数量级。我们相信所报道的方法可以为快速病毒识别提供可重复且具有成本效益的解决方案。
Here, we report the label-free, sensitive, and real-time electrical detection of whole viruses using carbon nanotube thin film (CNT-TF) field effect devices. Selective detection of approximately 550 model viruses, M13-bacteriophage, is demonstrated using a simple two-terminal (no gate electrode) configuration. Chemical gating through specific antibody-virus binding on CNT surface is proposed to be the sensing mechanism. Compared to electrical impedance sensors with identical microelectrode dimensions (no CNT), the CNT-TF sensors exhibit sensitivity 5 orders higher. We believe the reported approach could lead to a reproducible and cost-effective solution for rapid viral identification.
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