Whole genome sequence typing to investigate the Apophysomyces outbreak following a tornado in Joplin, Missouri, 2011.
Whole genome sequence typing to investigate the Apophysomyces outbreak following a tornado in Joplin, Missouri, 2011.
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DOI：
10.1371/journal.pone.0049989
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Engelthaler DM
中科院分区：
文献类型：
--
作者：
Etienne KA;Gillece J;Hilsabeck R;Schupp JM;Colman R;Lockhart SR;Gade L;Thompson EH;Sutton DA;Neblett-Fanfair R;Park BJ;Turabelidze G;Keim P;Brandt ME;Deak E;Engelthaler DM
附壁霉属的病例报告。在免疫活性宿主中的感染是由Apophysomyces spp.孢子污染的土壤或碎片。2011年5月22日，密苏里州乔普林市发生龙卷风，造成13名龙卷风受害者因空气传播物质的撕裂而感染了Apophysomycesalziformis。我们使用全基因组序列分型（WGST）对17个爆发的Apophysomyces分离株和另外5个时空多样的Apophysomyces对照分离株（3个A. A. ziziformis和两个A. variabilis isolates）。全基因组SNP系统发育分析显示，三个集群的基因型相关或相同的A。在乔普林组中，有多个不同的分离株;这表明来自单一或多个来源的多个基因型。虽然没有观察到基因型和暴露地点之间的联系，WGST分析确定，乔普林分离株之间的关系比对照分离株更密切，这表明当地的人口结构。此外，基于WGST的物种划分表明需要重新评估目前公认的Apophysomyces属内系统发育物种的分类学分类。
Case reports of Apophysomyces spp. in immunocompetent hosts have been a result of traumatic deep implantation of Apophysomyces spp. spore-contaminated soil or debris. On May 22, 2011 a tornado occurred in Joplin, MO, leaving 13 tornado victims with Apophysomyces trapeziformis infections as a result of lacerations from airborne material. We used whole genome sequence typing (WGST) for high-resolution phylogenetic SNP analysis of 17 outbreak Apophysomyces isolates and five additional temporally and spatially diverse Apophysomyces control isolates (three A. trapeziformis and two A. variabilis isolates). Whole genome SNP phylogenetic analysis revealed three clusters of genotypically related or identical A. trapeziformis isolates and multiple distinct isolates among the Joplin group; this indicated multiple genotypes from a single or multiple sources. Though no linkage between genotype and location of exposure was observed, WGST analysis determined that the Joplin isolates were more closely related to each other than to the control isolates, suggesting local population structure. Additionally, species delineation based on WGST demonstrated the need to reassess currently accepted taxonomic classifications of phylogenetic species within the genus Apophysomyces.
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影响因子：
1.9
作者：
Alvarez, Eduardo;Stchigel, Alberto M.;Guarro, Josep
通讯作者：
Guarro, Josep
影响因子：
4.9
作者：
Saravia-Flores, Marcos;Guaran, Dennya M.;Argueta, Victor
通讯作者：
Argueta, Victor
影响因子：
6.4
作者：
Hendriksen RS;Price LB;Schupp JM;Gillece JD;Kaas RS;Engelthaler DM;Bortolaia V;Pearson T;Waters AE;Upadhyay BP;Shrestha SD;Adhikari S;Shakya G;Keim PS;Aarestrup FM
通讯作者：
Aarestrup FM
影响因子：
5.4
作者：
Pearson T;Giffard P;Beckstrom-Sternberg S;Auerbach R;Hornstra H;Tuanyok A;Price EP;Glass MB;Leadem B;Beckstrom-Sternberg JS;Allan GJ;Foster JT;Wagner DM;Okinaka RT;Sim SH;Pearson O;Wu Z;Chang J;Kaul R;Hoffmaster AR;Brettin TS;Robison RA;Mayo M;Gee JE;Tan P;Currie BJ;Keim P
通讯作者：
Keim P
影响因子：
11.8
作者：
Engelthaler DM;Chiller T;Schupp JA;Colvin J;Beckstrom-Sternberg SM;Driebe EM;Moses T;Tembe W;Sinari S;Beckstrom-Sternberg JS;Christoforides A;Pearson JV;Carpten J;Keim P;Peterson A;Terashita D;Balajee SA
通讯作者：
Balajee SA