Small nucleolar RNAs in plasma extracellular vesicles and their discriminatory power as diagnostic biomarkers of Alzheimer's disease.
Small nucleolar RNAs in plasma extracellular vesicles and their discriminatory power as diagnostic biomarkers of Alzheimer's disease.
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DOI：
10.1016/j.nbd.2021.105481
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发表时间：
2021-11
影响因子：
6.1
通讯作者：
Lefterov, Iliya
中科院分区：
文献类型：
--
作者：
Fitz, Nicholas F.;Wang, Jiebiao;Kamboh, M. Ilyas;Koldamova, Radosveta;Lefterov, Iliya
阿尔茨海默病的早期临床诊断仍然是一项艰巨的任务。先进的成像技术和检测CSF中Aβ肽Aβ42和Aβ400总tau和磷酸化tau的实验室测定提供了一组发展中AD脑病理学的生物标志物，并促进了诊断过程。生物流体生物标志物的搜索，而不是在CSF中，和生物标志物检测的发展已显着加快，现在代表AD研究中增长最快的领域。本研究的目的是确定AD患者和认知正常对照（NC）血浆来源的细胞外囊泡（EV）中非编码RNA（ncRNA）的差异富集。使用RNA-seq，我们分析了四种重要的ncRNA：miRNA，snoRNA，tRNA和piRNA。我们报告了一个显着的富集SNORDs -一组snoRNA，在AD样品相比，NC。为了验证位于人类染色体15 q11-q13上的两个SNORDs簇-SNORD 115和SNORD 116的差异富集，我们使用了一组独立的AD患者和NC的血浆样本。我们应用ddPCR技术鉴定了SNORD 115和SNORD 116，它们具有高的区分能力来区分AD样品和NC样品。我们的研究结果表明，AD与血浆EV中从印迹基因组位点转录的SNORD富集的变化相关，并为进一步探索这些SNORD作为AD血浆生物标志物的有效性提供了理论基础。
The clinical diagnosis of Alzheimer’s disease, at its early stage, remains a difficult task. Advanced imaging technologies and laboratory assays to detect Aβ peptides Aβ42 and Aβ400 total and phosphorylated tau in CSF provide a set of biomarkers of developing AD brain pathology and facilitate the diagnostic process. The search for biofiuid biomarkers, other than in CSF, and the development of biomarker assays have accelerated significantly and now represent the fastest-growingfield in AD research. The goal of this study was to determine the differential enrichment of noncoding RNAs (ncRNAs) in plasma-derived extracellular vesicles (EV) of AD patients and Cognitively Normal controls (NC). Using RNA-seq, we profiled four signficant classes of ncRNAs: miRNAs, snoRNAs, tRNAs, and piRNAs. We report a significant enrichment of SNORDs – a group of snoRNAs, in AD samples compared to NC. To verify the differential enrichment of two clusters of SNORDs – SNORD115 and SNORD116, localized on human chromosome 15q11-q13, we used plasma samples of an independent group of AD patients and NC. We applied ddPCR technique and identified SNORD115 and SNORD116 with a high discriminatory power to differentiate AD samples from NC. The results of our study present evidence that AD is associated with changes in the enrichment of SNORDs, transcribed from imprinted genomic loci, in plasma EV and provide a rationale to further explore the validity of those SNORDs as plasma biomarkers of AD.
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DOI：
10.1007/s00259-021-05253-y
发表时间：
2021-07
影响因子：
9.1
作者：
Ashton NJ;Leuzy A;Karikari TK;Mattsson-Carlgren N;Dodich A;Boccardi M;Corre J;Drzezga A;Nordberg A;Ossenkoppele R;Zetterberg H;Blennow K;Frisoni GB;Garibotto V;Hansson O
通讯作者：
Hansson O
影响因子：
4
作者：
Chivet M;Hemming F;Pernet-Gallay K;Fraboulet S;Sadoul R
通讯作者：
Sadoul R
DOI：
10.1002/wrna.1150
发表时间：
2013-03
期刊：
Wiley interdisciplinary reviews. RNA
影响因子：
--
作者：
Chamberlain SJ
通讯作者：
Chamberlain SJ
影响因子：
6.4
作者：
Gao, Lu;Ma, Jie;Jiang, Feng
通讯作者：
Jiang, Feng
影响因子：
3.5
作者：
Irimie AI;Zimta AA;Ciocan C;Mehterov N;Dudea D;Braicu C;Berindan-Neagoe I
通讯作者：
Berindan-Neagoe I