In vitro expansion and differentiation of fresh and revitalized adult canine bone marrow-derived and adipose tissue-derived stromal cells.
In vitro expansion and differentiation of fresh and revitalized adult canine bone marrow-derived and adipose tissue-derived stromal cells.
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DOI：
10.1016/j.tvjl.2010.12.030
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发表时间：
2012-02
影响因子：
2.2
通讯作者：
Lopez, Mandi J.
中科院分区：
文献类型：
--
作者：
Spencer, Nakia D.;Chun, Raymond;Vidal, Martin A.;Gimble, Jeffrey M.;Lopez, Mandi J.
本研究的目的是确定犬脂肪组织来源的（ASC）和骨髓来源的（BMSC）基质细胞的组织密度，体外扩增和分化。在新鲜细胞中测定原代（P0）和细胞传代1-6（P1-6）细胞倍增数（CD）和倍增时间（DT）。在低温保存的细胞中评估P0、P3和P6成脂（CFU-Ad）、成骨（CFU-Ob）和成纤维细胞（CFU-F）集落形成单位频率、分化的P3细胞中的谱系特异性mRNA水平以及P3和P6成软骨颗粒的组成。骨髓细胞产量明显高于脂肪组织。总体ASC和BMSC CD和DT以及P3和P6 CFU-F、CFU-Ad和CFU-Ob相当。P0骨髓间充质干细胞CFU-Ob显著高于ASC。谱系特异性mRNA水平在分化细胞中高于对照细胞，但在细胞类型之间相似。P3与P6 ASC软骨形成颗粒中的蛋白质显著更高。基于这些发现，新鲜和再生的犬ASC是BMSC用于基质细胞应用的可行替代品。
The objective of this study was to determine the tissue density, in vitro expansion and differentiation of canine adipose tissue-derived (ASC) and bone marrow-derived (BMSC) stromal cells. Primary (P0) and cell passages 1–6 (P1–6) cell doubling numbers (CD) and doubling times (DT) were determined in fresh cells. The P0, P3, and P6 adipogenic (CFU-Ad), osteogenic (CFU-Ob), and fibroblastic (CFU-F) colony forming unit frequencies, lineage specific mRNA levels in differentiated P3 cells and composition of P3 and P6 chondrogenic pellets were assessed in cryogenically preserved cells. Cell yields from bone marrow were significantly higher than adipose tissue. Overall ASC and BMSC CDs and DTs and P3 and P6 CFU-F, CFU-Ad, and CFU-Ob were comparable. The P0 BMSC CFU-Ob was significantly higher than ASC. Lineage specific mRNA levels were higher in differentiated versus control cells, but similar between cell types. Protein was significantly greater in P3 versus P6 ASC chondrogenic pellets. Based on these findings, fresh and revitalized canine ASCs are viable alternatives to BMSCs for stromal cell applications.
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影响因子：
2.9
作者：
LABARCA, C;PAIGEN, K
通讯作者：
PAIGEN, K
影响因子：
5.2
作者：
Mitchell, James B.;McIntosh, Kevin;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.
DOI：
10.1038/oby.2004.55
发表时间：
2004-03-01
期刊：
OBESITY RESEARCH
影响因子：
--
作者：
Bakker, AHF;van Dielen, FMH;Buurman, WA
通讯作者：
Buurman, WA
影响因子：
--
作者：
Mochizuki, T;Muneta, T;Sekiya, I
通讯作者：
Sekiya, I
影响因子：
--
作者：
Jang S;Cho HH;Cho YB;Park JS;Jeong HS
通讯作者：
Jeong HS