Synteny analysis of genes and distribution of loci controlling oil content and fatty acid profile based on QTL alignment map in Brassica napus.
Synteny analysis of genes and distribution of loci controlling oil content and fatty acid profile based on QTL alignment map in Brassica napus.
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基于QTL比对图的甘蓝型油菜基因及控制油含量和脂肪酸谱的位点分布的同线性分析
DOI：
10.1186/s12864-017-4176-6
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发表时间：
2017-10-12
期刊：
影响因子：
4.4
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Raboanatahiry N;Chao H;Guo L;Gan J;Xiang J;Yan M;Zhang L;Yu L;Li M
背景：解读一个物种的遗传结构是了解其进化历史的一个好方法，也是为培育具有理想性状的优良品种而定制其概况的一个好方法。将不同群体的QTL定位在同一张图上，不仅可以用于比较，还可以作为多基因分析的基础，为了解与特定表型相关的遗传系统提供更有力的证据。结果：在本研究中，共鉴定出439个与脂肪酸（FA）和三酰甘油（TAG）生物合成相关的基因。B。与B相比，油菜基因组表现出混合的基因丢失和插入。rapa和B. C基因组插入基因较多。将9个已报道的油分和脂肪酸的QTL定位在参考基因组Darmor-bzh的物理图谱上，构建了油分和脂肪酸的QTL图谱。共检测到335个FA-QTLs和OC-QTLs，其中82个QTLs重叠。C3染色体含有22个重叠的QTL，除C18：3外，所有性状均被研究。在162个QTL的置信区间内共检测到218个与FA和TAG相关的候选基因，其中一些基因可能影响多个性状。其中76个候选基因位于57个重叠QTL内，以含油量候选基因占多数（61/76）。在3个群体中共检测到16个QTL重叠位点，在2个群体中共检测到60个QTL重叠位点。对这些候选基因的互作网络和途径分析表明，有10个基因可能对其它控制脂肪酸和油脂形成的基因有较强的影响。结论：本研究结果为B中FA和TAG形成的遗传基础提供了新的信息。油菜。构建了一张包含多个群体QTL的图谱，为B的基因组图谱研究提供了参考。napus，并且出现了可能影响种子油的新的潜在基因。为群体选择和/或感兴趣基因的筛选提供了新的有用途径。
Background:Deciphering the genetic architecture of a species is a good way to understand its evolutionary history, but also to tailor its profile for breeding elite cultivars with desirable traits. Aligning QTLs from diverse population in one map and utilizing it for comparison, but also as a basis for multiple analyses assure a stronger evidence to understand the genetic system related to a given phenotype.Results:In this study, 439 genes involved in fatty acid (FA) and triacylglycerol (TAG) biosyntheses were identified in Brassica napus. B. napus genome showed mixed gene loss and insertion compared to B. rapa and B. oleracea, and C genome had more inserted genes. Identified QTLs for oil (OC-QTLs) and fatty acids (FA-QTLs) from nine reported populations were projected on the physical map of the reference genome "Darmor-bzh" to generate a map. Thus, 335 FA-QTLs and OC-QTLs could be highlighted and 82 QTLs were overlapping. Chromosome C3 contained 22 overlapping QTLs with all trait studied except for C18:3. In total, 218 candidate genes which were potentially involved in FA and TAG were identified in 162 QTLs confidence intervals and some of them might affect many traits. Also, 76 among these candidate genes were found inside 57 overlapping QTLs, and candidate genes for oil content were in majority (61/76 genes). Then, sixteen genes were found in overlapping QTLs involving three populations, and the remaining 60 genes were found in overlapping QTLs of two populations. Interaction network and pathway analysis of these candidate genes indicated ten genes that might have strong influence over the other genes that control fatty acids and oil formation.Conclusion:The present results provided new information for genetic basis of FA and TAG formation in B. napus. A map including QTLs from numerous populations was built, which could serve as reference to study the genome profile of B. napus, and new potential genes emerged which might affect seed oil. New useful tracks were showed for the selection of population or/and selection of interesting genes for breeding improvement purpose.
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影响因子：
2.1
作者：
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