Sargassum fusiforme Polysaccharides Prevent High-Fat Diet-Induced Early Fasting Hypoglycemia and Regulate the Gut Microbiota Composition.
Sargassum fusiforme Polysaccharides Prevent High-Fat Diet-Induced Early Fasting Hypoglycemia and Regulate the Gut Microbiota Composition.
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DOI：
10.3390/md18090444
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发表时间：
2020-08-27
期刊：
影响因子：
5.4
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Wei B;Zhong QW;Ke SZ;Zhou TS;Xu QL;Wang SJ;Chen JW;Zhang HW;Jin WH;Wang H
空腹血糖低是糖尿病患者的常见症状，早期高脂饮食（HFD）喂养可导致空腹血糖低，这可能在糖尿病的发生发展中起重要作用，但很少受到关注。本研究从羊栖菜中提取了5种多糖，并研究了它们对HFD诱导的空腹低血糖和肠道菌群失调的影响。结果表明，C57 BL/6 J雄性小鼠喂食HFD 4周后发生严重的低血糖，由Sf-2、Sf-3、Sf-3-1和Sf-A组成的4种羊栖菜多糖（SFPs）显著预防早期空腹低血糖，而不诱导高血糖。Sf-1和Sf-A也能显著抑制HFD诱导的体重增加。Sf-2、Sf-3、Sf-3-1和Sf-A主要减弱HFD诱导的拟杆菌减少，所有五种SFP对颤螺旋体、粘螺菌和梭菌目的相对丰度有相当大的影响。相关分析表明，空腹血糖水平与粘螺旋体和颤螺旋体的相对丰度有关。受试者工作特征分析表明粘螺旋菌和颤螺旋菌对空腹低血糖有较好的预测能力（AUC = 0.745-0.833）。我们的研究结果突出了SFPs（特别是Sf-A）在葡萄糖稳态中的新应用以及粘螺旋菌和颤螺旋菌在SFPs生物活性中的潜在作用。
A low fasting blood glucose level is a common symptom in diabetes patients and can be induced by high-fat diet (HFD) feeding at an early stage, which may play important roles in the development of diabetes, but has received little attention. In this study, five polysaccharides were prepared from Sargassum fusiforme and their effects on HFD-induced fasting hypoglycemia and gut microbiota dysbiosis were investigated. The results indicated that C57BL/6J male mice fed an HFD for 4 weeks developed severe hypoglycemia and four Sargassum fusiforme polysaccharides (SFPs), consisting of Sf-2, Sf-3, Sf-3-1, and Sf-A, significantly prevented early fasting hypoglycemia without inducing hyperglycemia. Sf-1 and Sf-A could also significantly prevent HFD-induced weight gain. Sf-2, Sf-3, Sf-3-1, and Sf-A mainly attenuated the HFD-induced decrease in Bacteroidetes, and all five SFPs had a considerable influence on the relative abundance of Oscillospira, Mucispirillum, and Clostridiales. Correlation analysis revealed that the fasting blood glucose level was associated with the relative abundance of Mucispinllum and Oscillospira. Receiver operating characteristic analysis indicated that Mucispinllum and Oscillospira exhibited good discriminatory power (AUC = 0.745–0.833) in the prediction of fasting hypoglycemia. Our findings highlight the novel application of SFPs (especially Sf-A) in glucose homeostasis and the potential roles of Mucispinllum and Oscillospira in the biological activity of SFPs.
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影响因子：
5.4
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