RNAi-mediated stable silencing of TaCSN5 confers broad-spectrum resistance to Puccinia striiformis f. sp. tritici.
RNAi-mediated stable silencing of TaCSN5 confers broad-spectrum resistance to Puccinia striiformis f. sp. tritici.
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DOI：
10.1111/mpp.13034
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发表时间：
2021-04
影响因子：
4.9
通讯作者：
Guo J
中科院分区：
文献类型：
--
作者：
Bai X;Huang X;Tian S;Peng H;Zhan G;Goher F;Guo J;Kang Z;Guo J
组成型光形态发生9 （COP9）信号体（CSN）是植物生长、发育和对多种病原体反应的多功能调节剂。然而，很少有研究了解这些CSN基因在广谱抗病原体中的功能。本研究发现，接种小麦条纹状锈菌（Pst）和水杨酸（SA）处理后，TaCSN5基因的转录水平发生了变化。TaCSN5在拟南芥中的过表达导致对丁香假单胞菌pv的易感性增加。番茄DC3000感染后，AtPR1表达下调。在小麦品系中，过表达TaCSN5显著增加对Pst的敏感性，同时降低SA积累，而TaCSN5‐RNAi的小麦品系表现出相反的趋势。此外，我们发现TaCSN5负向调控参与SA信号通路的TaG3NPR1基因。此外，TaCSN5‐RNAi系对多种Pst的抗性增强。综上所述，我们证明了TaCSN5以SA依赖的方式参与小麦对Pst抗性的负调控。稳定的T4代转基因TaCSN5沉默植株对多品种条锈菌表现出广谱抗性。
The constitutive photomorphogenesis 9 (COP9) signalosome (CSN) is a versatile regulator of plant growth, development, and response to diverse pathogens. However, little research has been done to understand the function of those CSN genes in broad‐spectrum resistance to pathogens. In this study, we found that the transcript levels of wheat TaCSN5 were induced in response to inoculation with Puccinia striiformis f. sp. tritici (Pst) and treatment with salicylic acid (SA). Overexpression of TaCSN5 in Arabidopsis resulted in increased susceptibility to Pseudomonas syringae pv. tomato DC3000 infection accompanied by down‐regulation of AtPR1 expression. Overexpression of TaCSN5 in wheat lines significantly increased susceptibility to Pst accompanied by decreased SA accumulation, whereas TaCSN5‐RNAi wheat lines exhibited opposite trends. Moreover, we found that TaCSN5 negatively regulated TaG3NPR1 genes involved in the SA signalling pathway. In addition, TaCSN5‐RNAi lines showed increased resistance to multiple races of Pst. Taken together, we demonstrate that TaCSN5 contributes to negative regulation of wheat resistance to Pst in an SA‐dependent manner. Stable T4 generation transgenic TaCSN5‐silenced plants exhibited broad‐spectrum resistance to multiple races of stripe rust fungus.
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DOI：
10.1074/jbc.m112.352484
发表时间：
2012-08-24
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Emberley ED;Mosadeghi R;Deshaies RJ
通讯作者：
Deshaies RJ
小麦 Bax Inhibitor-1 蛋白与水通道蛋白 TaPIP1 相互作用并增强拟南芥的抗病性
影响因子：
5.6
作者：
Lu PP;Yu TF;Zheng WJ;Chen M;Zhou YB;Chen J;Ma YZ;Xi YJ;Xu ZS
通讯作者：
Xu ZS
影响因子：
5.6
作者：
Gao J;Bi W;Li H;Wu J;Yu X;Liu D;Wang X
通讯作者：
Wang X
影响因子：
4.9
作者：
Niki, T;Mitsuhara, I;Ohashi, Y
通讯作者：
Ohashi, Y
影响因子：
7.4
作者：
MAUCH, F;MAUCHMANI, B;BOLLER, T
通讯作者：
BOLLER, T