The antimicrobial resistance patterns and associated determinants in Streptococcus suis isolated from humans in southern Vietnam, 1997-2008.
The antimicrobial resistance patterns and associated determinants in Streptococcus suis isolated from humans in southern Vietnam, 1997-2008.
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DOI：
10.1186/1471-2334-11-6
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发表时间：
2011-01-06
影响因子：
3.7
通讯作者：
Schultsz C
中科院分区：
文献类型：
--
作者：
Hoa NT;Chieu TT;Nghia HD;Mai NT;Anh PH;Wolbers M;Baker S;Campbell JI;Chau NV;Hien TT;Farrar J;Schultsz C
猪链球菌是一种新兴的人畜共患病原体，是越南成人细菌性脑膜炎的主要原因。目前缺乏从人类病例中分离的猪链球菌菌株的抗微生物敏感性的系统数据。我们研究了从越南南部脑膜炎患者中分离的猪链球菌的抗菌素耐药性和相关耐药性决定因素。研究了1997 - 2008年分离的猪链球菌菌株对6种抗菌药物的敏感性。筛选菌株四环素和红霉素耐药决定因子的存在和表达，以及tet(M)基因与Tn916样转座子的关联。采用脉冲场凝胶电泳和Southern印迹法对四环素耐药基因tet(L)进行定位。我们观察到对四环素和氯霉素的耐药性显著增加，同时多药耐药增加。在四环素耐药菌株中，我们鉴定出tet(M)、tet(O)、tet(W)和tet(L)，并证实了它们的表达。所有tet(M)基因都与一个tn916样转座子相关。tet(L)和其他编码核糖体保护蛋白(s)的四环素耐药基因(s)的共表达仅在四环素最低抑制浓度(MIC)≥64 mg/L的菌株中检测到。我们证明，在研究的11年期间，越南南部引起人类疾病的猪链球菌的多药耐药性有所增加。我们报道了tet(L)在猪链球菌中的存在和表达，我们的数据表明，对于四环素，MIC≥64 mg/L需要多个编码不同机制的基因共同表达。
Streptococcus suis is an emerging zoonotic pathogen and is the leading cause of bacterial meningitis in adults in Vietnam. Systematic data on the antimicrobial susceptibility profiles of S. suis strains isolated from human cases are lacking. We studied antimicrobial resistance and associated resistance determinants in S. suis isolated from patients with meningitis in southern Vietnam. S. suis strains isolated between 1997 and 2008 were investigated for their susceptibility to six antimicrobial agents. Strains were screened for the presence and expression of tetracycline and erythromycin resistance determinants and the association of tet(M) genes with Tn916- like transposons. The localization of tetracycline resistance gene tet(L) was determined by pulse field gel electrophoresis and Southern blotting. We observed a significant increase in resistance to tetracycline and chloramphenicol, which was concurrent with an increase in multi-drug resistance. In tetracycline resistance strains, we identified tet(M), tet(O), tet(W) and tet(L) and confirmed their expression. All tet(M) genes were associated with a Tn916-like transposon. The co-expression of tet(L) and other tetracycline resistance gene(s) encoding for ribosomal protection protein(s) was only detected in strains with a minimum inhibitory concentration (MIC) of tetracycline of ≥ 64 mg/L We demonstrated that multi-drug resistance in S. suis causing disease in humans in southern Vietnam has increased over the 11-year period studied. We report the presence and expression of tet(L) in S. suis strains and our data suggest that co-expression of multiple genes encoding distinct mechanism is required for an MIC ≥ 64 mg/L to tetracycline.
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影响因子：
3.3
作者：
Ng, LK;Martin, I;Mulvey, M
通讯作者：
Mulvey, M
影响因子：
11.8
作者：
Nghia, Ho Dang Trung;Hoa, Ngo Thi;Linh, Le Dieu;Campbell, James;Diep, To Song;Chau, Nguyen Van Vinh;Mai, Nguyen Thi Hoang;Hien, Tran Tinh;Spratt, Brian;Farrar, Jeremy;Schultsz, Constance
通讯作者：
Schultsz, Constance
影响因子：
3.3
作者：
Martel, A;Baele, M;Haesebrouck, F
通讯作者：
Haesebrouck, F
影响因子：
1.5
作者：
CANTIN, M;HAREL, J;GOTTSCHALK, M
通讯作者：
GOTTSCHALK, M
DOI：
10.1016/s0924-8579(03)00062-1
发表时间：
2003-06-01
影响因子：
10.8
作者：
Rodriguez-Avial, I;Rodriguez-Avial, C;Picazo, JJ
通讯作者：
Picazo, JJ