Identification of AFLP fragments linked to hydroxysafflor yellow A in Flos Carthami and conversion to a SCAR marker for rapid selection
Identification of AFLP fragments linked to hydroxysafflor yellow A in Flos Carthami and conversion to a SCAR marker for rapid selection
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红花中与羟基红花黄色 A 连锁的 AFLP 片段的鉴定并转换为 SCAR 标记以进行快速选择
DOI：
10.1007/s11032-008-9228-9
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发表时间：
2009-02
影响因子：
3.1
通讯作者：
中科院分区：
文献类型：
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作者：
羟基红花黄色素A（HSYA）是红花（Carthamus tinctorius）花瓣中特有的一种治疗局灶性心脑缺血的活性物质，本研究对红花HSYA性状进行了遗传和分子标记分析。用高效液相色谱法分析了亲本、杂交F1和F2代的HSYA含量。结果表明，HSYA的存在/不存在是由一个主要的核基因HSya控制的。共筛选出48对AFLP引物组合，并从F2分离群体的498个个体中选出10个HSYA存在株和10个HSYA缺失株，制备两个池进行混合分离分析。结果表明，AFLP-5、AFLP-7、AFLP-15和AFLP-16是与HSya紧密相关的4个AFLP标记。其中AFLP-16与HSya的遗传距离最近，估计为9.4cM。根据AFLP片段侧翼克隆区的序列信息，将显性AFLP-16标记转化为简单的序列特征扩增区标记，命名为SCM 16。本研究结果对红花品质育种的分子标记辅助选择具有直接的应用价值。
Hydroxysafflor yellow A (HSYA), an important active compound in treating focal cardiac and cerebral ischemia, is uniquely present in flower petals ofCarthamus tinctorius.In this study, inheritance and molecular marker analyses for HSYA trait in safflower were carried out. HSYA contents in parents, cross hybridized F1and F2individuals were analyzed by high performance liquid chromatography. Results revealed that the presence/absence of HSYA was controlled by one major nuclear gene termedHSya. A total of 48 AFLP primer combinations were screened, and bulked segregant analysis was performed by preparing two pools of 10 present-HSYA and ten absent-HSYA plants selected from the 498 individuals of the F2segregating population. Four AFLP markers, AFLP-5, AFLP-7, AFLP-15 and AFLP-16, were identified to be closely associated withHSya. Of those, AFLP-16 was the closest toHSya, estimated at about 9.4 cM in genetic distance. The dominant AFLP-16 marker was converted into a simple sequence characterized amplified region marker based on the sequence information of the cloned flanking regions of the AFLP fragment and was designated as SCM16. Our result has direct application for marker-assisted selection of quality breeding in safflower.
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DOI：
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发表时间：
2004-10
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
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作者：
Jia Luo;Zhi-ping Fang;Li-Ming Zhou;Song Lai
通讯作者：
Jia Luo;Zhi-ping Fang;Li-Ming Zhou;Song Lai
影响因子：
3.1
作者：
Naweed I. Naqvi;Bharat B. Chattoo
通讯作者：
Naweed I. Naqvi;Bharat B. Chattoo
影响因子：
4.3
作者：
Y. Siow;P. Choy;W. Leung;O. Karmin
通讯作者：
Y. Siow;P. Choy;W. Leung;O. Karmin
DOI：
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发表时间：
2004-05
期刊：
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
影响因子：
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作者：
Ming Jin;Jinrong Li;Wei Wu
通讯作者：
Ming Jin;Jinrong Li;Wei Wu
影响因子：
1.7
作者：
Yu-zhi Zhou;Hong-yu Ma;Huan Chen;Li Qiao;Yao Yao-Yao;Jian-Qing Cao;Y. Pei
通讯作者：
Yu-zhi Zhou;Hong-yu Ma;Huan Chen;Li Qiao;Yao Yao-Yao;Jian-Qing Cao;Y. Pei