Tick capillary feeding for the study of proteins involved in tick-pathogen interactions as potential antigens for the control of tick infestation and pathogen infection.
Tick capillary feeding for the study of proteins involved in tick-pathogen interactions as potential antigens for the control of tick infestation and pathogen infection.
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DOI：
10.1186/1756-3305-7-42
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发表时间：
2014-01-22
影响因子：
3.2
通讯作者：
de la Fuente J
中科院分区：
文献类型：
--
作者：
Antunes S;Merino O;Mosqueda J;Moreno-Cid JA;Bell-Sakyi L;Fragkoudis R;Weisheit S;Pérez de la Lastra JM;Alberdi P;Domingos A;de la Fuente J
蜱对动物和人类的健康构成重大风险，因为它们在进食过程中可以传播多种病原体。传统上使用化学品控制蜱有严重的缺点，包括选择抗杀螨剂的蜱和化学残留物对环境的污染。用蜱中肠抗原BM 86进行疫苗接种被证明是牛蜱控制的良好替代方案。然而，结果在蜱种和地理位置之间差异很大。因此，需要新的抗原来开发控制蜱虫侵染和病原体感染/传播的疫苗。蜱-病原体相互作用中涉及的蜱蛋白可能为这些疫苗提供良好的候选保护性抗原，但需要适当的筛选程序来选择最佳候选抗原。在这项研究中，我们选择了参与蜱-无形体（Subolesin和SILK）和蜱-巴贝虫（TROSPA）相互作用的蛋白质，并使用体外毛细血管饲养来表征其作为控制牛蜱侵染和感染边缘无形体和二联巴贝虫的抗原的潜力。产生针对重组牛蜱、SILK和TROSPA的纯化兔多克隆抗体，并将其添加到未感染或感染的牛血液中，以毛细管饲养雌性微小扇头蜱（牛蜱）。在处理和对照蜱中测定蜱重、产卵和病原体DNA水平。纯化的兔多克隆抗体对蜱重组蛋白的特异性通过Western印迹和对蜱细胞系和蜱组织中的天然蛋白质使用免疫荧光来确认。毛细管喂食的蜱虫摄取了添加到血粉中的抗体，并显示了这些抗体对蜱虫重量和产卵的影响。然而，对病原体DNA水平没有观察到影响。这些结果突出了体外蜱毛细血管摄食用于表征候选蜱保护性抗原的优点和一些缺点。虽然观察到对蜱虫重量和产卵的影响，但对病原体水平的影响并不明显，可能是由于其他因素中蜱虫间的差异较大。尽管如此，这些结果与先前的RNA干扰功能研究结果一起表明，这些蛋白是控制R.微小线虫侵染和A. marginale和B.二联。
Ticks represent a significant health risk to animals and humans due to the variety of pathogens they can transmit during feeding. The traditional use of chemicals to control ticks has serious drawbacks, including the selection of acaricide-resistant ticks and environmental contamination with chemical residues. Vaccination with the tick midgut antigen BM86 was shown to be a good alternative for cattle tick control. However, results vary considerably between tick species and geographic location. Therefore, new antigens are required for the development of vaccines controlling both tick infestations and pathogen infection/transmission. Tick proteins involved in tick-pathogen interactions may provide good candidate protective antigens for these vaccines, but appropriate screening procedures are needed to select the best candidates. In this study, we selected proteins involved in tick-Anaplasma (Subolesin and SILK) and tick-Babesia (TROSPA) interactions and used in vitro capillary feeding to characterize their potential as antigens for the control of cattle tick infestations and infection with Anaplasma marginale and Babesia bigemina. Purified rabbit polyclonal antibodies were generated against recombinant SUB, SILK and TROSPA and added to uninfected or infected bovine blood to capillary-feed female Rhipicephalus (Boophilus) microplus ticks. Tick weight, oviposition and pathogen DNA levels were determined in treated and control ticks. The specificity of purified rabbit polyclonal antibodies against tick recombinant proteins was confirmed by Western blot and against native proteins in tick cell lines and tick tissues using immunofluorescence. Capillary-fed ticks ingested antibodies added to the blood meal and the effect of these antibodies on tick weight and oviposition was shown. However, no effect was observed on pathogen DNA levels. These results highlighted the advantages and some of the disadvantages of in vitro tick capillary feeding for the characterization of candidate tick protective antigens. While an effect on tick weight and oviposition was observed, the effect on pathogen levels was not evident probably due to high tick-to-tick variations among other factors. Nevertheless, these results together with previous results of RNA interference functional studies suggest that these proteins are good candidate vaccine antigens for the control of R. microplus infestations and infection with A. marginale and B. bigemina.
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影响因子：
5.7
作者：
Hajdušek O;Síma R;Ayllón N;Jalovecká M;Perner J;de la Fuente J;Kopáček P
通讯作者：
Kopáček P
影响因子：
2.1
作者：
Macaluso, Kevin;Sonenshine, Daniel;Azad, Abdu
通讯作者：
Azad, Abdu
影响因子：
2.6
作者：
de la Fuente, Jose;Moreno-Cid, Juan A.;Blouin, Edmour F.
通讯作者：
Blouin, Edmour F.
影响因子：
2.4
作者：
Jongejan, F;Uilenberg, G
通讯作者：
Uilenberg, G
影响因子：
2.5
作者：
de la Fuente, Jose;Almazan, Consuelo;Willadsen, Peter
通讯作者：
Willadsen, Peter