Engineered tissue micro-rings fabricated from aggregated fibroblasts and microfibres for a bottom-up tissue engineering approach
Engineered tissue micro-rings fabricated from aggregated fibroblasts and microfibres for a bottom-up tissue engineering approach
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由聚集的成纤维细胞和微纤维制成的工程组织微环，用于自下而上的组织工程方法
DOI：
10.1088/1758-5090/ab1ee5
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发表时间：
2019-06
期刊：
影响因子：
9
通讯作者：
Fukuda Toshio
中科院分区：
文献类型：
--
作者：
Sun Tao;Shi Qing;Yao Yibing;Sun Junzhong;Wang Huaping;Huang Qiang;Fukuda Toshio
具有并入的微支架的组织环已被设计为用于自下而上构建生物管的有前途的构建块。然而，目前可用于掺入的微支架非常有限。在本文中，我们提供了一种有效的策略，首先将微流控纺海藻酸钙微纤维封装磁性纳米粒子到自组装成纤维细胞微环。基于表面修饰，尺寸为40 μm的微纤维允许成纤维细胞沿长轴沿着扩散和增殖。通过评估培养3天后成纤维细胞在微纤维上的覆盖程度来获得最佳细胞接种密度。然后，我们设计了一个磁引导培养装置与多个环形微孔，以促进细胞驱动的组装的微纤维。依赖于表面张力的操作策略用于在细胞接种之前使微纤维沿着微孔图案化，并且磁吸引进一步防止图案化的微纤维在培养期间沉积在微孔中。在培养的3天内，在微孔中形成并入微纤维的组织微环。形成过程的定量分析揭示了液体样聚集行为，并纳入微纤维显示出促进组织微环中细胞定向组织的潜力。此外，磁驱动操作被机器人地用于将微环组装在插入培养装置中心的微柱上。在培养5天以允许细胞融合后，获得生物管状微结构。微流体纺丝可以产生具有各种形状，几何形状和组成的纤维;因此，我们提出的方法极大地丰富了可用于并入组织环的各种微支架，以设计用于组织工程和再生医学的复杂人工器官。
Tissue rings with incorporated microscaffolds have been engineered as promising building blocks for constructing biological tubes from the bottom up. However, the microscaffolds available for incorporation are very limited at present. In this paper we provide an efficient strategy to first incorporate microfluidic spun Ca-alginate microfibres encapsulating magnetic nanoparticles into self-assembled fibroblast micro-rings. Based on the surface modification, microfibres with a size of ∼40 μm allowed fibroblasts to spread and proliferate along the long axis. The optimal cell seeding density was obtained by evaluating the degree of coverage of fibroblasts on microfibres after 3 days of culture. Then we designed a magnetically guided culture apparatus with multiple annular micro-wells to facilitate cell-driven assembly of microfibres. A manipulation strategy dependent on surface tension was used to pattern microfibres along the micro-wells prior to cell seeding, and magnetic attraction further kept the patterned microfibres from being deposited in the micro-wells during cultivation. Within 3 days of culture, microfibre-incorporated tissue micro-rings were formed in the micro-wells. Quantitative analysis of the formation process revealed liquid-like aggregating behaviours, and incorporated microfibres showed the potential to promote the directed organization of cells in tissue micro-rings. Furthermore, magnetically driven manipulation was used robotically to assemble the micro-rings on a micropillar inserted into the centre of the culture apparatus. After 5 days of culture to allow cell fusion, a biological tubular microstructure was achieved. Microfluidic spinning can generate fibres with a variety of shapes, geometries, and compositions; therefore, our proposed method greatly enriches the variety of microscaffolds available for incorporation into tissue rings to engineer complex artificial organs for tissue engineering and regenerative medicine.
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影响因子：
14
作者：
Norotte, Cyrille;Marga, Francois S.;Niklason, Laura E.;Forgacs, Gabor
通讯作者：
Forgacs, Gabor
影响因子：
9.7
作者：
Ju, Young Min;Ahn, Hyunhee;Lee, Sang Jin
通讯作者：
Lee, Sang Jin
影响因子：
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作者：
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通讯作者：
Khademhosseini A
磁性藻酸盐微纤维作为支架元件用于制造微血管样结构
影响因子：
9.7
作者：
Sun Tao;Shi Qing;Huang Qiang;Wang Huaping;Xiong Xiaolu;Hu Chengzhi;Fukuda Toshio
通讯作者：
Fukuda Toshio
影响因子：
14
作者：
He, Wei;Nieponice, Alejandro;Soletti, Lorenzo;Hong, Yi;Gharaibeh, Burhan;Crisan, Mihaela;Usas, Arvydas;Peault, Bruno;Huard, Johnny;Wagner, William R.;Vorp, David A.
通讯作者：
Vorp, David A.