Isolation and functional gene analyses of aromatic-hydrocarbon-degrading bacteria from a polychlorinated-dioxin-dechlorinating process.
Isolation and functional gene analyses of aromatic-hydrocarbon-degrading bacteria from a polychlorinated-dioxin-dechlorinating process.
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DOI：
10.1264/jsme2.me11283
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发表时间：
2012
影响因子：
2.2
通讯作者：
Hiraishi A
中科院分区：
文献类型：
--
作者：
Kaiya S;Utsunomiya S;Suzuki S;Yoshida N;Futamata H;Yamada T;Hiraishi A
以二苯并呋喃为模型底物，通过富集和平板培养，从一个对多氯二苯并对二恶英/二苯并呋喃(PCDD/FS)有明显完全脱氯作用的半厌氧微生物微生态中分离到好氧芳烃降解菌。通过16S rRNA基因序列比对，这些降解二苯并呋喃的菌株被鉴定为放线杆菌门、细长杆菌门和变形杆菌门的成员，其中以拟青霉属和根瘤菌的含量最高。所有菌株均以萘为唯一碳源和能源，以代谢或共代谢方式降解二苯并呋喃，但对一氯二苯并呋喃和二苯并-对二恶英几乎不产生攻击作用。通过PCR克隆和测序，在所有测试菌株中都检测到了编码芳香环羟化双加氧酶(AhDO)的基因。实时荧光定量聚合酶链式反应检测到约105个拷贝的大亚基基因g−1湿重在获得分离株的微宇宙中。这个拷贝数的顺序对应于来自“脱氢球菌”及其近亲作为有效脱氯剂存在的约1%的16S rRNA基因拷贝。这些结果表明，在PCDD/F-脱氯过程中，好氧细菌共存，并在少氯和完全脱氯产物的氧化降解中发挥作用，从而实现了PCDD/FS明显的完全脱氯。
Aerobic aromatic-hydrocarbon-degrading bacteria from a semi-anaerobic microbial microcosm that exhibited apparent complete dechlorination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) were isolated through enrichment and plating culture procedures with dibenzofuran as the model substrate. By 16S rRNA gene sequence comparisons, these dibenzofuran-degrading isolates were identified as being members of the phyla Actinobacteria, Firmicutes, and Proteobacteria, among which those of the genera Paenibacillus and Rhizobium were most abundant. All of the isolates utilized naphthalene as the sole carbon and energy source and degraded dibenzofuran metabolically or co-metabolically; however, they hardly attacked monochlorinated dibenzofuran and dibenzo-p-dioxin. By PCR cloning and sequencing, genes predicted to encode aromatic-ring-hydroxylating dioxygenase (AhDO) were detected in all test isolates. Real-time quantitative PCR assays with specific primer sets detected approximately 105 copies of the AhDO large subunit genes g−1 wet wt in the microcosm from which the isolates were obtained. This order of the copy number corresponded to approximately 1% of the 16S rRNA gene copies from “Dehalococcoides” and its relatives present as potent dechlorinators. These results suggest that aerobic AhDO-containing bacteria co-exist and play a role in the oxidative degradation of less chlorinated and completely dechlorinated products in the PCDD/F-dechlorinating process, thereby achieving the apparent complete dechlorination of PCDD/Fs.
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DOI：
10.1099/00207713-52-1-131
发表时间：
2002-01-01
影响因子：
2.8
作者：
Daane, LL;Harjono, I;Häggblom, MM
通讯作者：
Häggblom, MM
影响因子：
2.6
作者：
Hunter, W. J.;Kuykendall, L. D.;Manter, D. K.
通讯作者：
Manter, D. K.
影响因子：
2.8
作者：
Hiraishi, A;Yonemitsu, Y;Kawahara, K
通讯作者：
Kawahara, K
影响因子：
11
作者：
Futamata, Hiroyuki;Yoshida, Naoko;Hiraishi, Akira
通讯作者：
Hiraishi, Akira
影响因子：
11.4
作者：
Fennell, DE;Nijenhuis, I;Häggblom, MM
通讯作者：
Häggblom, MM