Phospholipids fatty acids analysis of microbial communities in sewage sludge composting with inorganic bulking agent
Phospholipids fatty acids analysis of microbial communities in sewage sludge composting with inorganic bulking agent
复制标题
无机填充剂堆肥污泥中微生物群落磷脂脂肪酸分析
DOI：
10.1080/19443994.2016.1173594
复制
发表时间：
2016-04
影响因子：
1.1
通讯作者：
Li Weiguang
中科院分区：
文献类型：
--
作者：
Wu Chu;ong;Wang Wei;Wang Ke;Li Xiangkun;Qiu Wei;Li Weiguang
通过对污泥中磷脂脂肪酸（PLFAs）的分析，研究了污泥堆肥100 d过程中微生物群落的变化。采用浮石作为无机填充剂，消除了常规有机物对污泥PLFAs分析结果的影响。结果表明，在第10天出现最大PLFA含量407.41 μg g−1VS，在第100天检测到最小PLFA含量207.49 μg g−1VS。高温阶段的微生物以细菌和真菌为主。在腌制过程中，细菌数量明显减少，而革兰氏阳性菌（14：0 iso，16：0 iso，17：0 anteiso）、假单胞菌（18：1ω 7 c）、放线菌（18：0 10-甲基）和真菌（18：3ω6c（6，9，12））的丰度同时趋于增加。原生动物在原污泥和成熟样品中检测到，但在嗜热期内没有出现。采用培养依赖法验证微生物种群结果。细菌和真菌的数量在第10天达到最大值（分别为3.38 × 1012和3.31 × 109 CFU g-1d-s），然后下降。放线菌的数量逐渐增加，在第100天达到3.24 × 107 CFU g-1d s。结果表明，PLFAs技术是一种有效、简便的研究污泥堆肥过程中微生物群落变化的方法。
Phospholipids fatty acids (PLFAs) in sewage sludge were analyzed to evaluate the variation in the microbial communities during 100 d composting. Pumice was used as an inorganic bulking agent to eliminate the influences of conventional organic materials on the PLFAs analysis result of the sludge. The results showed that the maximal PLFAs content of 407.41 μg g−1VS was presented on Day-10 and the minimal PLFAs content of 207.49 μg g−1VS was detected on Day-100. Bacteria and fungi were mainly the micro-organisms in the thermophilic stage. The number of bacteria distinctly reduced during the curing stage, whereas the abundances of gram-positive bacteria (14:0 iso, 16:0 iso, 17:0 anteiso), pseudomona (18:1ω7c), actinobacteria (18:0 10-methyl), and fungi (18:3ω6c (6, 9, 12)) tended to increase simultaneously. Protozoa were detected in the raw sludge and the matured sample, but not presented within the theromophilic period. Culture-dependent method was introduced to verify the results of microbial populations. The numbers of bacteria and fungi reached maximum (3.38 × 1012and 3.31 × 109CFU g−1d s, respectively) on Day-10 and then reduced. The number of actinomycetes increased gradually and reached 3.24 × 107CFU g−1d s, on Day-100. The results indicated that PLFAs technique is an efficient and convenient method to investigate the variation in the microbial communities during sewage sludge composting.
登录
查看更多内容
DOI：
10.2488/jwrs.54.289
发表时间：
2008
期刊：
Journal of the Japan Wood Research Society
影响因子：
--
作者：
M. Matsushita;Ayami Hato;S. Meguro
通讯作者：
M. Matsushita;Ayami Hato;S. Meguro
影响因子：
2.4
作者：
L. Wu;L. Ma;G. Martínez
通讯作者：
L. Wu;L. Ma;G. Martínez
影响因子：
2.1
作者：
Schaefer, Jenny;Jaeckel, Udo;Kaempfer, Peter
通讯作者：
Kaempfer, Peter
DOI：
10.1016/j.ibiod.2010.01.012
发表时间：
2010-10-01
影响因子：
4.8
作者：
Amir, Soumia;Abouelwafa, Rajae;Hafidi, Mohamed
通讯作者：
Hafidi, Mohamed
影响因子：
9.7
作者：
C. Liang;R. Fujinuma;T. Balser
通讯作者：
C. Liang;R. Fujinuma;T. Balser