Diaphorin, a Polyketide Produced by a Bacterial Symbiont of the Asian Citrus Psyllid, Inhibits the Growth and Cell Division of Bacillus subtilis but Promotes the Growth and Metabolic Activity of Escherichia coli.
Diaphorin, a Polyketide Produced by a Bacterial Symbiont of the Asian Citrus Psyllid, Inhibits the Growth and Cell Division of Bacillus subtilis but Promotes the Growth and Metabolic Activity of Escherichia coli.
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DOI：
10.1128/spectrum.01757-22
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发表时间：
2022-08-31
影响因子：
3.7
通讯作者：
Nakabachi, Atsushi
中科院分区：
文献类型：
--
作者：
Tanabe, Nozomu;Takasu, Rena;Hirose, Yuu;Kamei, Yasuhiro;Kondo, Maki;Nakabachi, Atsushi
Diaphorin是由“Armatura Profftella”（γ变形菌门：伯克霍尔德菌目）产生的聚酮化合物，其是臭名昭著的农业害虫亚洲柑橘木虱Diaphorina citri（半翅目：木虱科）的专性共生体。黄递素属于pederin家族的生物活性剂中发现的各种主机共生系统，包括甲虫，地衣，和海绵，窝藏遗传多样性的细菌生产者。以往的研究表明，存在于D。citri在2 - 20 mM的浓度下，对各种真核生物，包括D.柑橘。然而，很少有人知道它对原核生物的影响。为了解决这个问题，本研究评估了黄递素对两种模式原核生物，大肠杆菌（γ-变形菌门：肠球菌目）和枯草芽孢杆菌（厚壁菌门：芽孢杆菌）的生物活性。采用分光光度法、光学显微镜、图像分析和透射电镜对它们的生长和形态特征进行了分析。E.使用β-半乳糖苷酶测定进一步评估大肠杆菌。结果表明，生理浓度的黄递素抑制了B的生长和细胞分裂。枯草芽孢杆菌（E. subtilis）的生长和代谢活性，但对E.杆菌这一发现意味着，黄递蛋白作为一种防御剂的holobiont（主机加共生体）对一些细菌谱系，但代谢有益的其他人，其中可能包括专性共生体的D。柑橘。某些次级代谢物，包括抗生素，进化为介导生物体之间的相互作用。这些分子对微生物具有不同的光谱，并且通常对革兰氏阳性细菌比革兰氏阴性细菌更有效。然而，单个分子对不同的细菌谱系具有完全相反的活性是罕见的。本研究揭示了木虱的细胞器样细菌共生体合成的次级代谢产物抑制革兰氏阳性枯草芽孢杆菌的生长，但促进革兰氏阴性大肠杆菌的生长。这一发现不仅提供了对动物宿主中微生物组进化的见解，而且可能被利用来促进微生物工业材料生产的有效性。
Diaphorin is a polyketide produced by “Candidatus Profftella armatura” (Gammaproteobacteria: Burkholderiales), an obligate symbiont of a notorious agricultural pest, the Asian citrus psyllid Diaphorina citri (Hemiptera: Psyllidae). Diaphorin belongs to the pederin family of bioactive agents found in various host-symbiont systems, including beetles, lichens, and sponges, harboring phylogenetically diverse bacterial producers. Previous studies showed that diaphorin, which is present in D. citri at concentrations of 2 to 20 mM, has inhibitory effects on various eukaryotes, including the natural enemies of D. citri. However, little is known about its effects on prokaryotic organisms. To address this issue, the present study assessed the biological activities of diaphorin on two model prokaryotes, Escherichia coli (Gammaproteobacteria: Enterobacterales) and Bacillus subtilis (Firmicutes: Bacilli). Their growth and morphological features were analyzed using spectrophotometry, optical microscopy followed by image analysis, and transmission electron microscopy. The metabolic activity of E. coli was further assessed using the β-galactosidase assay. The results revealed that physiological concentrations of diaphorin inhibit the growth and cell division of B. subtilis but promote the growth and metabolic activity of E. coli. This finding implies that diaphorin functions as a defensive agent of the holobiont (host plus symbionts) against some bacterial lineages but is metabolically beneficial for others, which potentially include obligate symbionts of D. citri. IMPORTANCE Certain secondary metabolites, including antibiotics, evolve to mediate interactions among organisms. These molecules have distinct spectra for microorganisms and are often more effective against Gram-positive bacteria than Gram-negative ones. However, it is rare that a single molecule has completely opposite activities on distinct bacterial lineages. The present study revealed that a secondary metabolite synthesized by an organelle-like bacterial symbiont of psyllids inhibits the growth of Gram-positive Bacillus subtilis but promotes the growth of Gram-negative Escherichia coli. This finding not only provides insights into the evolution of microbiomes in animal hosts but also may potentially be exploited to promote the effectiveness of industrial material production by microorganisms.
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影响因子：
12.3
作者：
Gerardo NM;Altincicek B;Anselme C;Atamian H;Barribeau SM;de Vos M;Duncan EJ;Evans JD;Gabaldón T;Ghanim M;Heddi A;Kaloshian I;Latorre A;Moya A;Nakabachi A;Parker BJ;Pérez-Brocal V;Pignatelli M;Rahbé Y;Ramsey JS;Spragg CJ;Tamames J;Tamarit D;Tamborindeguy C;Vincent-Monegat C;Vilcinskas A
通讯作者：
Vilcinskas A
影响因子：
4
作者：
Arp AP;Hunter WB;Pelz-Stelinski KS
通讯作者：
Pelz-Stelinski KS
影响因子：
3.7
作者：
Dan H;Ikeda N;Fujikami M;Nakabachi A
通讯作者：
Nakabachi A
DOI：
10.1073/pnas.92.21.9465
发表时间：
1995-10-10
影响因子：
11.1
作者：
LEMAITRE, B;KROMERMETZGER, E;HOFFMANN, JA
通讯作者：
HOFFMANN, JA
影响因子：
4
作者：
de Leon, L.;Moujir, L.
通讯作者：
Moujir, L.