Ultrasensitive electrochemical genosensors for species-specific diagnosis of malaria.
Ultrasensitive electrochemical genosensors for species-specific diagnosis of malaria.
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DOI：
10.1016/j.electacta.2022.140988
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发表时间：
2022-10-10
影响因子：
6.6
通讯作者：
Kanyong, Prosper
中科院分区：
文献类型：
--
作者：
Ansah, Felix;Krampa, Francis;Donkor, Jacob K.;Owusu-Appiah, Caleb;Ashitei, Sarah;Kornu, Victor E.;Danku, Reinhard K.;Chirawurah, Jersley D.;Awandare, Gordon A.;Aniweh, Yaw;Kanyong, Prosper
关键词：
电化学基因传感器被开发用于疟疾的物种特异性诊断。该基因传感器是第一个用于检测三日疟和卵形疟原虫的生物传感器。疟疾基因传感器的检测限在阿托摩尔范围内。基因传感器可以直接检测寄生虫核酸，无需预扩增。基因传感器的性能与实时 PCR 相当。在护理点 (POC) 缺乏可靠的针对疟疾的物种特异性诊断工具仍然是有效疾病管理的重大障碍。部分原因是当前疟疾POC诊断试剂盒的敏感性和特异性有限，特别是在低密度寄生虫血症和混合物种感染的情况下。在这项研究中，我们描述了第一个基于电化学阻抗谱 (EIS) 的无标记 DNA 基因传感器，用于恶性疟原虫、三日疟原虫和卵形疟原虫的物种特异性检测。三种物种特异性基因传感器的检测限 (LOD) 降低至阿摩尔浓度，范围为 18.7 aM 至 43.6 aM，低于之前报道的疟疾基因传感器的检测限。更重要的是，将三种基因传感器的诊断性能与使用纯化的基因组 DNA 和来自临床样本的配对全血裂解物进行的定量实时聚合酶链反应 (qPCR) 测定进行了比较。值得注意的是，所有 qPCR 阳性的纯化基因组 DNA 样本均被基因传感器正确识别，表明对三种疟疾中的每一种都具有 100% 的灵敏度。三种基因传感器的特异性范围为 66.7% 至 100.0%，治疗周转时间 (TTAT) 在 30 分钟内，与当前疟疾 POC 诊断工具的 TTAT 相当。这项工作代表着朝着开发准确、快速的物种特异性核酸工具包迈出的重要一步，该工具包用于在 POC 诊断疟疾。
Electrochemical genosensors were developed for species-specific diagnosis of malaria. The genosensors are the first biosensors for detecting P. malariae and P. ovale. The limits of detection of the malaria genosensors are in the attomolar range. The genosensors can directly detect parasite nucleic acid without pre-amplification. The performance of the genosensors is comparable to real-time PCR. The absence of reliable species-specific diagnostic tools for malaria at point-of-care (POC) remains a major setback towards effective disease management. This is partly due to the limited sensitivity and specificity of the current malaria POC diagnostic kits especially in cases of low-density parasitaemia and mixed species infections. In this study, we describe the first label-free DNA-based genosensors based on electrochemical impedance spectroscopy (EIS) for species-specific detection of P. falciparum, P. malariae and P. ovale. The limits of detection (LOD) for the three species-specific genosensors were down in attomolar concentrations ranging from 18.7 aM to 43.6 aM, which is below the detection limits of previously reported malaria genosensors. More importantly, the diagnostic performance of the three genosensors were compared to quantitative real-time polymerase chain reaction (qPCR) assays using purified genomic DNA and the paired whole blood lysates from clinical samples. Remarkably, all the qPCR-positive purified genomic DNA samples were correctly identified by the genosensors indicating 100% sensitivity for each of the three malaria species. The specificities of the three genosensors ranged from 66.7% to 100.0% with a Therapeutic Turnaround Time (TTAT) within 30 min, which is comparable to the TTAT of current POC diagnostic tools for malaria. This work represents a significant step towards the development of accurate and rapid species-specific nucleic acid-based toolkits for the diagnosis of malaria at the POC.
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影响因子：
3.2
作者：
Hawadak J;Dongang Nana RR;Singh V
通讯作者：
Singh V
DOI：
10.1016/j.jmoldx.2021.07.022
发表时间：
2021-10
期刊：
The Journal of molecular diagnostics : JMD
影响因子：
--
作者：
Ansah F;Suurbaar J;Darko D;Anabire NG;Blankson SO;Domson BKS;Soulama A;Kpasra P;Chirawurah JD;Amenga-Etego L;Kanyong P;Awandare GA;Aniweh Y
通讯作者：
Aniweh Y
影响因子：
5.3
作者：
Dembele, Laurent;Diallo, Nouhoum;Sogore, Fanta;Diarra, Bintou;Ballo, Fatoumata, I;Daou, Amadou;Diakite, Ousmaila;Bare, Yacouba;Sangare, Cheick Papa Oumar;Haidara, Aboubecrin Sedhigh;Diakite, Seidina A. S.;Niangaly, Amadou;Diakite, Mahamadou;Campo, Brice;Awandare, Gordon A.;Aniweh, Yaw;Djimde, Abdoulaye A.
通讯作者：
Djimde, Abdoulaye A.
影响因子：
5.4
作者：
Butterworth, Adrian;Blues, Elizabeth;Corrigan, Damion K.
通讯作者：
Corrigan, Damion K.
在赤道几内亚的代表性样本中检测三种诊断方法（显微镜，RDT和PCR）的三种诊断方法（显微镜，RDT和PCR）的比较。
影响因子：
3
作者：
Berzosa P;de Lucio A;Romay-Barja M;Herrador Z;González V;García L;Fernández-Martínez A;Santana-Morales M;Ncogo P;Valladares B;Riloha M;Benito A
通讯作者：
Benito A