Response of Aquatic Bacterial Communities to Hydraulic Fracturing in Northwestern Pennsylvania: A Five-Year Study.
Response of Aquatic Bacterial Communities to Hydraulic Fracturing in Northwestern Pennsylvania: A Five-Year Study.
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DOI：
10.1038/s41598-018-23679-7
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发表时间：
2018-04-09
影响因子：
4.6
通讯作者：
Lamendella R
中科院分区：
文献类型：
--
作者：
Ulrich N;Kirchner V;Drucker R;Wright JR;McLimans CJ;Hazen TC;Campa MF;Grant CJ;Lamendella R
水平钻井和水力压裂开采程序在宾夕法尼亚州越来越普遍，马塞勒斯页岩油气藏主要位于宾夕法尼亚州。长期环境影响的潜力，附近的上游河流生态系统和水生细菌组合仍然不完全了解。在这里，我们对16 S rRNA基因进行了高通量测序，以表征水，沉积物和其他环境样品（n = 189）的细菌群落结构，这些样品来自宾夕法尼亚州西北部五年（2012-2016）中表现出不同历史的水力压裂活动的31个源头流站点。溪流pH值被确定为溪流中细菌变化的主要驱动因素，而水力压裂活动则充当溪流沉积物中某些较低分类水平成员的环境选择器。与MSA−流相比，在显示Marcellus页岩活性（MSA+）的站点中，甲烷营养菌和产甲烷菌（即甲基囊菌科、拜杰林基菌科和甲烷杆菌属）显著富集。这项研究强调了与新生Marcellus页岩活动相关的潜在哨兵类群，其中一些类群在这项为期五年的研究中仍然是稳定的生物标志物。确定压裂影响的溪流中特定微生物群落的存在和功能将更清楚地了解天然微生物群落对压裂的反应，并为原位修复策略提供信息。
Horizontal drilling and hydraulic fracturing extraction procedures have become increasingly present in Pennsylvania where the Marcellus Shale play is largely located. The potential for long-term environmental impacts to nearby headwater stream ecosystems and aquatic bacterial assemblages is still incompletely understood. Here, we perform high-throughput sequencing of the 16 S rRNA gene to characterize the bacterial community structure of water, sediment, and other environmental samples (n = 189) from 31 headwater stream sites exhibiting different histories of fracking activity in northwestern Pennsylvania over five years (2012–2016). Stream pH was identified as a main driver of bacterial changes within the streams and fracking activity acted as an environmental selector for certain members at lower taxonomic levels within stream sediment. Methanotrophic and methanogenic bacteria (i.e. Methylocystaceae, Beijerinckiaceae, and Methanobacterium) were significantly enriched in sites exhibiting Marcellus shale activity (MSA+) compared to MSA− streams. This study highlighted potential sentinel taxa associated with nascent Marcellus shale activity and some of these taxa remained as stable biomarkers across this five-year study. Identifying the presence and functionality of specific microbial consortia within fracking-impacted streams will provide a clearer understanding of the natural microbial community’s response to fracking and inform in situ remediation strategies.
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DOI：
10.1098/rspb.2008.0284
发表时间：
2008-07-22
影响因子：
4.7
作者：
Davies, T. Jonathan;Pedersen, Amy B.
通讯作者：
Pedersen, Amy B.
影响因子：
2.3
作者：
Brablcova, Lenka;Buriankova, Iva;Rulik, Martin
通讯作者：
Rulik, Martin
影响因子：
2.7
作者：
Grant, Christopher James;Lutz, Allison K.;Stanton, Mitchell R.
通讯作者：
Stanton, Mitchell R.
影响因子：
48
作者：
Edgar, Robert C.
通讯作者：
Edgar, Robert C.
DOI：
10.1073/pnas.0507535103
发表时间：
2006-01-17
影响因子：
11.1
作者：
Fierer, N;Jackson, RB
通讯作者：
Jackson, RB