A virus-like particle enzyme-linked immunosorbent assay detects serum antibodies in a majority of women infected with human papillomavirus type 16.
A virus-like particle enzyme-linked immunosorbent assay detects serum antibodies in a majority of women infected with human papillomavirus type 16.
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DOI：
10.1093/jnci/86.7.494
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发表时间：
1994-04-06
期刊：
影响因子：
--
通讯作者：
Schiller JT
中科院分区：
文献类型：
--
作者：
Kirnbauer R;Hubbert NL;Wheeler CM;Becker TM;Lowy DR;Schiller JT
以前的研究表明，生殖器感染高危类型的人乳头瘤病毒(HPV)，最常见的是HPV16，是宫颈癌发展的最重要的风险因素。然而，已开发的用于识别高危HPV感染的血清学分析要么未能将血清反应与HPV感染的其他指标联系起来，要么仅确定了少数HPV感染者。我们的目的是确定一种专门建立的酶联免疫吸附试验(ELISA)是否可以检测经DNA检测为生殖器HPV16感染阳性的妇女血清中的抗HPV16病毒颗粒抗体。以新研制的HPV16病毒样颗粒为抗原，建立了检测抗HPV16病毒粒子抗体的酶联免疫吸附试验。这些颗粒由HPV16结构蛋白组成，由重组杆状病毒表达后在昆虫细胞中自我组装。用该酶联免疫吸附试验检测了122例既往已用核酸方法检测HPV状态的妇女血清。聚合酶链式反应(PCR)检测生殖器HPV16DNA阳性的妇女中，有59%(32/54)的血清在酶联免疫吸附试验中呈阳性，而HPVDNA阴性的妇女血清中HPV16DNA检测为阴性(P<；.0005)。相比之下，根据这一标准，6%的HPVDNA阴性妇女(2/31)和9%的低风险HPV6/11DNA阳性妇女(1/11)是ELISA阳性。对另外两种高危HPV类型的DNA阳性的妇女的血清进行了评估；HPV18阳性的妇女中有31%(4/13)和HPV31阳性的妇女中有38%(5/13)的血清在HPV16EL ISA中呈阳性。在HPV16DNA阳性的重度不典型增生妇女中，75%(12/16)的血清EL ISA结果为阳性。同时检测HPV16DNA的妇女中，67%(28/42)的血清在酶联免疫吸附试验中检测为阳性，相比之下，33%(4/12)的妇女(4/12)的血清经PCR检测为阳性，而病毒类型检测为阴性(P<；0.05)。大多数宫颈HPV16感染的妇女对HPV16L1的构象依赖表位产生一种免疫球蛋白抗体反应，这种抗体可以通过酶联免疫吸附试验检测到。这种特殊的或类似的含有其他HPV类型的病毒样颗粒的ELISA，可能有助于确定高危HPV感染的自然病史，或许有助于识别有患宫颈癌风险的女性。
Previous studies have demonstrated that genital infection with high-risk types of human papillomavirus (HPV), most often HPV16, is the most significant risk factor for the development of cervical cancer. However, serologic assays that have been developed to identify high-risk HPV infection have either failed to associate serum reactivity with other indicators of HPV infection or have identified only a minority of HPV-infected individuals. Our purpose was to determine whether a specifically developed enzyme-linked immunosorbent assay (ELISA) could detect IgG anti-HPV16 virion antibodies in the sera of women who had tested positive for genital HPV16 infection by DNA-based methods. An ELISA was developed using newly developed HPV16 virus-like particles as antigens to detect anti-HPV16 virion IgG antibodies. These particles are comprised of HPV16 structural proteins that are self-assembled in insect cells after expression by recombinant baculo-viruses. The sera of 122 women, whose HPV status had been previously evaluated by nucleic acid-based methods, were tested by this ELISA. The sera of 59% of women (32 of 54) positive for genital HPV16 DNA by polymerase chain reaction (PCR) were positive in the ELISA assay compared with sera from women who had tested negative for HPV DNA (P<.0005). In contrast, 6% of HPV DNA-negative women (two of 31) and 9% of women positive for low-risk HPV6/11 DNA (one of 11) were ELISA positive by this criterion. The sera of women who were DNA positive for two additional high-risk HPV types were evaluated; the sera of 31% of HPV18-positive (four of 13) and 38% of HPV31-positive women (five of 13) were positive in the HPV16 particle ELISA. The sera of 75% of HPV16 DNA-positive women with severe dysplasias (12 of 16) gave positive ELISA results. The sera of 67% of women (28 of 42) who tested positive for HPV16 DNA by both PCR and the less sensitive ViraType assay tested positive in the ELISA compared with 33% of women (four of 12) who were positive by PCR but negative by ViraType (P<.05). The majority of women with cervical HPV16 infection generate an IgG antibody response to conformationally dependent epitopes of HPV16 L1 that can be detected by ELISA. This particular ELISA, or a similar one incorporating virus-like particles of additional HPV types, may be useful in determining the natural history of high-risk HPV infection and perhaps help to identify women at risk for developing cervical cancer.
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影响因子：
3.1
作者：
WHEELER, CM;PARMENTER, CA;MANOS, MM
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