FvMYB79 Positively Regulates Strawberry Fruit Softening via Transcriptional Activation of FvPME38.
FvMYB79 Positively Regulates Strawberry Fruit Softening via Transcriptional Activation of FvPME38.
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FvMYB79 通过 FvPME38 的转录激活积极调节草莓果实软化
DOI：
10.3390/ijms23010101
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发表时间：
2021-12-22
影响因子：
5.6
通讯作者：
Xue C
中科院分区：
文献类型：
--
作者：
Cai J;Mo X;Wen C;Gao Z;Chen X;Xue C
草莓是一种柔软的水果，由于硬度迅速丧失，采后寿命很短。果胶甲酯酶（PME）介导的细胞壁重塑对决定果实的硬度和软化是重要的。在此之前，我们已经验证了FvPME38在PME介导的草莓果实软化的调节中的重要作用。然而，PME介导的果实软化的调控网络在很大程度上仍然是未知的。在这里，我们确定了一个R2R3型MYB转录因子FvMYB79，它激活FvPME38的表达水平，从而加速果实软化。在果实发育过程中，FvMYB79与FvPME38共表达，这种共表达模式与“Ruegen”果实硬度的变化相反，“Ruegen”果实硬度在果实发育过程中显著下降，转色期后突然变低。FvMYB79通过瞬时转化可显著提高FvPME38的转录水平，从而降低果实硬度，促进果实成熟。此外，沉默的FvMYB79表现出对ABA诱导的果实成熟的不敏感性，这表明FvMYB79可能参与ABA依赖的果实软化过程。我们的研究结果表明，FvMYB79作为一个新的调节器在草莓成熟过程中通过转录激活FvPME38，这提供了一个新的机制，为提高草莓果实硬度。
Strawberry is a soft fruit with short postharvest life, due to a rapid loss of firmness. Pectin methylesterase (PME)-mediated cell wall remodeling is important to determine fruit firmness and softening. Previously, we have verified the essential role of FvPME38 in regulation of PME-mediated strawberry fruit softening. However, the regulatory network involved in PME-mediated fruit softening is still largely unknown. Here, we identified an R2R3-type MYB transcription factor FvMYB79, which activates the expression level of FvPME38, thereby accelerating fruit softening. During fruit development, FvMYB79 co-expressed with FvPME38, and this co-expression pattern was opposite to the change of fruit firmness in the fruit of ‘Ruegen’ which significantly decreased during fruit developmental stages and suddenly became very low after the color turning stage. Via transient transformation, FvMYB79 could significantly increase the transcriptional level of FvPME38, leading to a decrease of firmness and acceleration of fruit ripening. In addition, silencing of FvMYB79 showed an insensitivity to ABA-induced fruit ripening, suggesting a possible involvement of FvMYB79 in the ABA-dependent fruit softening process. Our findings suggest FvMYB79 acts as a novel regulator during strawberry ripening via transcriptional activation of FvPME38, which provides a novel mechanism for improvement of strawberry fruit firmness.
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影响因子：
6.7
作者：
Dorokhov YL;Komarova TV;Petrunia IV;Frolova OY;Pozdyshev DV;Gleba YY
通讯作者：
Gleba YY
DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
影响因子：
4.1
作者：
Hasunuma, T;Fukusaki, E;Kobayashi, A
通讯作者：
Kobayashi, A
采后草莓果实对外源生长素和脱落酸的转录组分析
影响因子：
4.3
作者：
Chen, Jingxin;Mao, Linchun;Luo, Zisheng
通讯作者：
Luo, Zisheng
影响因子：
5.3
作者：
Dheilly E;Gall SL;Guillou MC;Renou JP;Bonnin E;Orsel M;Lahaye M
通讯作者：
Lahaye M