Alterations and abnormal mitosis of wheat chromosomes induced by wheat-rye monosomic addition lines.
Alterations and abnormal mitosis of wheat chromosomes induced by wheat-rye monosomic addition lines.
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小麦-黑麦单体附加系诱导的小麦染色体的改变和异常有丝分裂
DOI：
10.1371/journal.pone.0070483
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Tang Z
中科院分区：
文献类型：
--
作者：
Fu S;Yang M;Fei Y;Tan F;Ren Z;Yan B;Zhang H;Tang Z
背景小麦-黑麦附加系是一个古老的话题。然而，小麦-黑麦单体附加系引起的小麦染色体变异和有丝分裂异常的报道很少。方法/主要发现四倍体小黑麦是从普通小麦T. aestivum L. '绵阳11' ×黑麦S.燕麦属利用小黑麦与绵阳11号进行控制回交，然后自交，获得了‘Kustro’及部分后代。以黑麦基因组DNA为探针进行基因组原位杂交（GISH），以pAs 1和pSc119.2为探针进行荧光原位杂交（FISH），对这些后代的有丝分裂染色体进行分析。在‘绵阳11号’的3DS染色体臂的端粒区，可以观察到较强的pSc119.2 FISH信号。而4 R单体附加系自交后代的pSc119.2FISH信号消失，改变后的3D染色体能稳定地传递给下一代。在7 R单体附加系的自交后代中，观察到1条2D染色体断裂，3条4A染色体。在6 R单体异附加系自交后代中发现了3D染色体结构变异和有丝分裂行为异常。此外，6 R单体附加系的部分后代中，1A和4 B染色体被消除。这些结果表明，外源黑麦染色体可能引起小麦染色体的改变和有丝分裂行为的异常，外源黑麦染色体引起的胁迫可能是引起小麦核型改变的因素之一。
Background Wheat-rye addition lines are an old topic. However, the alterations and abnormal mitotic behaviours of wheat chromosomes caused by wheat-rye monosomic addition lines are seldom reported. Methodology/Principal Findings Octoploid triticale was derived from common wheat T. aestivum L. ‘Mianyang11’×rye S. cereale L. ‘Kustro’ and some progeny were obtained by the controlled backcrossing of triticale with ‘Mianyang11’ followed by self-fertilization. Genomic in situ hybridization (GISH) using rye genomic DNA and fluorescence in situ hybridization (FISH) using repetitive sequences pAs1 and pSc119.2 as probes were used to analyze the mitotic chromosomes of these progeny. Strong pSc119.2 FISH signals could be observed at the telomeric regions of 3DS arms in ‘Mianyang11’. However, the pSc119.2 FISH signals were disappeared from the selfed progeny of 4R monosomic addition line and the changed 3D chromosomes could be transmitted to next generation stably. In one of the selfed progeny of 7R monosomic addition line, one 2D chromosome was broken and three 4A chromosomes were observed. In the selfed progeny of 6R monosomic addition line, structural variation and abnormal mitotic behaviour of 3D chromosome were detected. Additionally, 1A and 4B chromosomes were eliminated from some of the progeny of 6R monosomic addition line. Conclusions/Significance These results indicated that single rye chromosome added to wheat might cause alterations and abnormal mitotic behaviours of wheat chromosomes and it is possible that the stress caused by single alien chromosome might be one of the factors that induced karyotype alteration of wheat.
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影响因子：
3.8
作者：
de las Heras, JI;King, IP;Parker, JS
通讯作者：
Parker, JS
影响因子：
3.1
作者：
Ma, Xue-Feng;Gustafson, J. Perry
通讯作者：
Gustafson, J. Perry
影响因子：
6.2
作者：
Brasileiro-Vidal, AC;Brammer, S;Guerra, M
通讯作者：
Guerra, M
影响因子：
5.4
作者：
Bento, Miguel;Gustafson, Perry;Silva, Manuela
通讯作者：
Silva, Manuela
影响因子：
2.6
作者：
Alkhimova, AG;Heslop-Harrison, JS;Vershinin, AV
通讯作者：
Vershinin, AV