Molecular detection of hepatitis E virus in sheep from southern Xinjiang, China.
Molecular detection of hepatitis E virus in sheep from southern Xinjiang, China.
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南疆绵羊戊型肝炎病毒分子检测
DOI：
10.1007/s11262-015-1194-9
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发表时间：
2015-06
期刊：
影响因子：
1.6
通讯作者：
Jin, Meilin
中科院分区：
文献类型：
--
作者：
Wu, Junyuan;Si, Fusheng;Jiang, Chunyu;Li, Tao;Jin, Meilin
戊型肝炎病毒（HEV）是发展中国家和发达国家动物和人类传染性肝炎的病原体。本研究从新疆南部某屠宰场采集了500份羊血清和75份生羊肝样品，沿着采集了26份屠宰工人血清。采用酶联免疫吸附试验检测血清抗-HEV抗体。通过巢式聚合酶链反应靶向开放阅读框2（ORF 2）的部分核苷酸序列，评价血清和肝脏样本中HEV RNA的存在。结果表明，500只绵羊中有176只血清阳性，阳性率为35.20%; 75只绵羊肝脏中有4只（5.3%）检出HEVRNA。屠夫的血清阳性率为57.7%（15/26）。4个扩增子的核苷酸序列同源性为97.8 ~ 100%，与HEV 1、2、3、4型相应区域的核苷酸序列同源性分别为81.6- 85.3%、84.2- 85.3%、82.1- 85.3%和84.7- 97.9%。基于扩增的186 bp ORF 2序列和相应参考菌株的比对构建系统发育树。结果表明，4个绵羊品系在基因型4簇内形成一个谱系，其中hb-3、bjsw 1、T1、swCH 189和swCH 25均属于基因型4，亚型4d。结果表明，在绵羊中的高水平的血清转化，并建议羊肝可能是一个来源的食源性戊型肝炎病毒感染的人。
Hepatitis E virus (HEV) is a causative agent of infectious hepatitis in animals and humans both in developing and developed countries. Here, we collected 500 sheep sera and 75 raw sheep liver samples from a slaughterhouse in the southern part of the Xinjiang region, China, along with 26 sera of butchers from the same slaughterhouse. All serum samples were tested for anti-HEV antibody by enzyme-linked immunosorbent assay. Both serum and liver samples were evaluated for the presence of HEV RNA by nested polymerase chain reaction targeting partial nucleotide sequences of open reading frame 2 (ORF2). The results indicate that sheep seroprevalence was 35.20 % (176/500) and that four of the 75 (5.3 %) sheep livers showed detectable amounts of HEV RNA. The seroprevalence of the butchers was 57.7 % (15/26). The four amplicons shared 97.8–100 % nucleotide sequence identity and had pairwise sequence identities of 81.6–85.3 %, 84.2–85.3 %, 82.1–85.3 % and 84.7–97.9 % with the corresponding regions of genotypes 1, 2, 3 and 4 of HEV, respectively. A phylogenetic tree was constructed based on alignments of an amplified 186-bp ORF2 sequence and corresponding reference strains. The analysis showed that the four sheep strains detected in our study formed a lineage within a genotype 4 cluster that contains hb-3, bjsw1, T1, swCH189 and swCH25, all of which belong to genotype 4, subtype 4d. The results indicated a high level of seroconversion in sheep and suggested that sheep liver may be a source of foodborne HEV infection in humans.
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