(18)F-fluorodeoxyglucose uptake and tumor hypoxia: revisit (18)f-fluorodeoxyglucose in oncology application.
(18)F-fluorodeoxyglucose uptake and tumor hypoxia: revisit (18)f-fluorodeoxyglucose in oncology application.
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DOI：
10.1016/j.tranon.2014.02.010
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发表时间：
2014-04
影响因子：
5
通讯作者：
Civelek, A. Cahid
中科院分区：
文献类型：
--
作者：
Li, Xiao-Feng;Du, Yang;Ma, Yuanyuan;Postel, Gregory C.;Civelek, A. Cahid
这项研究重新审视了各种肿瘤模型中 18F-氟脱氧葡萄糖 (18F-FDG) 的摄取及其与缺氧的关系。方法：我们在裸鼠体内制备了结直肠癌 HT29、乳腺癌 MDA-MB-231 和非小细胞肺癌 A549 细胞系的腹膜癌和皮下异种移植物。测量腹水的氧分压（pO2）。通过数字放射自显影检测到的 18F-FDG 积累与冰冻肿瘤切片中哌莫硝唑结合和葡萄糖转运蛋白 1 (GLUT-1) 可视化的肿瘤缺氧有关。结果：腹水 pO2 为 0.90 ± 0.53 mm Hg。悬浮在腹水中的单个癌细胞和簇以及亚毫米浆膜肿瘤的哌莫硝唑和 GLUT-1 染色呈阳性，并且具有高 18F-FDG 摄取。相比之下，较大浆膜肿瘤或皮下异种移植物的常氧部分（少量哌莫硝唑结合或GLUT-1表达）的18F-FDG摄取显着较低，与肝脏中的摄取没有统计学差异。结论：严重缺氧腹水癌和较大肿瘤缺氧部分的葡萄糖需求（18F-FDG 摄取）显着高于含氧量正常的癌细胞。最初从艾利希腹水癌中获得的瓦尔堡效应可能不适用于含氧量正常的癌细胞。我们的研究结果可能有助于更好地了解 18F-FDG PET 在肿瘤学中的应用。
This study revisited 18F-fluorodeoxyglucose (18F-FDG) uptake and its relationship to hypoxia in various tumor models. METHODS: We generated peritoneal carcinomatosis and subcutaneous xenografts of colorectal cancer HT29, breast cancer MDA-MB-231, and non–small cell lung cancer A549 cell lines in nude mice. The partial oxygen pressure (pO2) of ascites fluid was measured. 18F-FDG accumulation detected by digital autoradiography was related to tumor hypoxia visualized by pimonidazole binding and glucose transporter-1 (GLUT-1) in frozen tumor sections. RESULTS: Ascites pO2 was 0.90 ± 0.53 mm Hg. Single cancer cells and clusters suspended in ascites fluid as well as submillimeter serosal tumors stained positive for pimonidazole and GLUT-1 and had high 18F-FDG uptake. In contrast, 18F-FDG uptake was significantly lower in normoxic portion (little pimonidazole binding or GLUT-1 expression) of larger serosal tumors or subcutaneous xenografts, which was not statistically different from that in the liver. CONCLUSIONS: Glucose demand (18F-FDG uptake) in severely hypoxic ascites carcinomas and hypoxic portion of larger tumors is significantly higher than in normoxic cancer cells. Warburg effect originally obtained from Ehrlich ascites carcinoma may not apply to normoxic cancer cells. Our findings may benefit the better understanding of 18F-FDG PET in oncology application.
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影响因子：
5.3
作者：
Iwano, Shingo;Ito, Shinji;Naganawa, Shinji
通讯作者：
Naganawa, Shinji
影响因子：
5.7
作者：
Nordsmark, M;Overgaard, M;Overgaard, J
通讯作者：
Overgaard, J
影响因子：
11.2
作者：
Li, Xiao-Feng;Carlin, Sean;O'Donoghue, Joseph A.
通讯作者：
O'Donoghue, Joseph A.
某些人类肺癌的组织学结构以及对放射疗法的可能影响。
影响因子：
8.8
作者：
THOMLINSON, R H;GRAY, L H
通讯作者：
GRAY, L H
DOI：
10.1007/s00259-009-1310-y
发表时间：
2010-02
影响因子：
9.1
作者：
Li, Xiao-Feng;Sun, Xiaorong;Ma, Yuanyuan;Suehiro, Makiko;Zhang, Mutian;Russell, James;Humm, John L.;Ling, C. Clifton;O'Donoghue, Joseph A.
通讯作者：
O'Donoghue, Joseph A.