The BIG gene controls size of shoot apical meristems in Arabidopsis thaliana

The BIG gene controls size of shoot apical meristems in Arabidopsis thaliana
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BIG 基因控制拟南芥茎尖分生组织的大小

DOI:
10.1007/s00299-020-02510-6
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发表时间:
2020-02
期刊:
影响因子:
6.2
通讯作者:
Li Ping Tang
Li Ping Tang
中科院分区:
生物学2区
文献类型:
--
作者:
Wen Jie Zhang;Li Ming Zhai;Hai Xia Yu;Jing Peng;Shan Shan Wang;Xian Sheng Zhang;Ying Hua Su;Li Ping Tang

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BIG调节茎干细胞群体。茎顶端分生组织(SAM)是植物地上部分发生和发育的子细胞,是一个自我更新的细胞群。然而,SAM大小调节的潜在机制在很大程度上仍不清楚。在这里,我们确定了一个突变体,显示一个大的SAM,指定的大芽分生组织(大-M),在拟南芥。big-m的表型是由BIG的一个新的T-DNA插入等位基因引起的,导致功能丧失。大-m突变体在SAM中比野生型有更多的干细胞。与野生型相比,在big-m中WUSCHEL(WUS)和SHOOTMERISTEMLESS(STM)的表达得到促进,表明BIG在WUS和STM的上游起作用。因此,BIG是SAM中干细胞群的重要调节因子。此外,遗传分析表明,BIG与PIN-FORMED 1(PIN 1)在控制SAM大小方面协同作用。我们的研究结果表明,BIG通过PIN 1介导的生长素稳态在控制拟南芥SAM生长中起着重要作用。
BIG regulates the shoot stem cell population. The shoot apical meristem (SAM) contains a population of self-renewing cells, and provides daughter cells for initiation and development of aerial parts of plants. However, the underlying mechanisms of SAM size regulation remain largely unclear. Here, we identified a mutant that displayed a large SAM, designated big-shoot meristem (big-m), in Arabidopsis thaliana. The phenotype of big-m is caused by a new T-DNA insertion allele of BIG, causing a loss of function. The big-m mutant had more stem cells in the SAM than in the wild type. Expression of WUSCHEL (WUS) and SHOOTMERISTEMLESS (STM) was promoted in big-m compared with the wild type, showing that BIG functions upstream of WUS and STM. Therefore, BIG is an important regulator of the stem cell population in the SAM. Furthermore, genetic analysis indicated that BIG acts synergistically with PIN-FORMED1 (PIN1) in controlling SAM size. Our results suggest that BIG plays an important role in controlling Arabidopsis thaliana SAM growth via PIN1-mediated auxin homeostasis.
DOI: 10.1126/science.283.5409.1911
发表时间: 1999-03-19
期刊: SCIENCE
影响因子: 56.9
作者:
Fletcher, LC;Brand, U;Meyerowitz, EM
通讯作者: Meyerowitz, EM
DOI: --
发表时间: --
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期刊: CURRENT BIOLOGY
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DOI: 10.1016/j.pbi.2005.09.010
发表时间: 2005-12
影响因子: 9.5
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BIG 调节拟南芥的气孔免疫和茉莉酸产生
DOI: 10.1111/nph.15568
发表时间: 2019
期刊: NEW PHYTOLOGIST
影响因子: 9.4
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