The Putative C(2)H(2) Transcription Factor VadH Governs Development, Osmotic Stress Response, and Sterigmatocystin Production in Aspergillus nidulans.
The Putative C(2)H(2) Transcription Factor VadH Governs Development, Osmotic Stress Response, and Sterigmatocystin Production in Aspergillus nidulans.
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作者：
VosA-VelB异源二聚体复合物在构巢曲霉的发育和次生代谢调控中起着关键作用。在这项工作中，我们描述了一个新的VosA/VelB激活的基因称为vadH，这是预测编码一个457个氨基酸长度的蛋白质含有四个相邻的C2 H2锌指结构域。vosA或VelB的突变失活导致整个生命周期中vadH的mRNA水平降低，表明VosA和VelB对vadH的表达具有积极的调节作用。vadH的缺失导致无性发育（分生孢子）减少，但有性子实体（闭囊藻）的产量增加，表明VadH在A. nidulans。此外，与野生型相比，vadH缺失突变体表现出对高渗胁迫的敏感性升高，并表现出真菌毒素杂色曲霉素（ST）的产量升高。采用RNA-Seq的全基因组表达分析表明，VadH可能参与调节比营养生长阶段更多的基因和生物学途径。在vadH无效突变体中，分生孢子形成的中心调控途径brlA、abaA和wetA基因在无性发育过程中显著下调。VadH还参与调节mat 2、ppgA和lsdA等基因，与性发育有关，以及ST生物合成基因簇中的一些基因。综上所述，VadH是一个具有4个C2 H2指状结构域的转录因子，参与调节A. nidulans。
The VosA-VelB hetero-dimeric complex plays a pivotal role in regulating development and secondary metabolism in Aspergillus nidulans. In this work, we characterize a new VosA/VelB-activated gene called vadH, which is predicted to encode a 457-amino acid length protein containing four adjacent C2H2 zinc-finger domains. Mutational inactivation of vosA or velB led to reduced mRNA levels of vadH throughout the lifecycle, suggesting that VosA and VelB have a positive regulatory effect on the expression of vadH. The deletion of vadH resulted in decreased asexual development (conidiation) but elevated production of sexual fruiting bodies (cleistothecia), indicating that VadH balances asexual and sexual development in A. nidulans. Moreover, the vadH deletion mutant exhibited elevated susceptibility to hyperosmotic stress compared to wild type and showed elevated production of the mycotoxin sterigmatocystin (ST). Genome-wide expression analyses employing RNA-Seq have revealed that VadH is likely involved in regulating more genes and biological pathways in the developmental stages than those in the vegetative growth stage. The brlA, abaA, and wetA genes of the central regulatory pathway for conidiation are downregulated significantly in the vadH null mutant during asexual development. VadH also participates in regulating the genes, mat2, ppgA and lsdA, etc., related to sexual development, and some of the genes in the ST biosynthetic gene cluster. In summary, VadH is a putative transcription factor with four C2H2 finger domains and is involved in regulating asexual/sexual development, osmotic stress response, and ST production in A. nidulans.
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DOI：
10.1007/978-1-62703-122-6_13
发表时间：
2012-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Park, Hee Soo;Yu, Jae-Hyuk
通讯作者：
Yu, Jae-Hyuk
影响因子：
--
作者：
Slattery, MG;Liko, D;Heideman, W
通讯作者：
Heideman, W
影响因子：
2.5
作者：
Zhao, Yanxia;Lee, Mi-Kyung;Yu, Jae-Hyuk
通讯作者：
Yu, Jae-Hyuk
影响因子：
1.9
作者：
Yu JH
通讯作者：
Yu JH
影响因子：
4.5
作者：
Sarikaya Bayram O;Bayram O;Valerius O;Park HS;Irniger S;Gerke J;Ni M;Han KH;Yu JH;Braus GH
通讯作者：
Braus GH