Diversity in the major polysaccharide antigen of Acinetobacter baumannii assessed by DNA sequencing, and development of a molecular serotyping scheme.
Diversity in the major polysaccharide antigen of Acinetobacter baumannii assessed by DNA sequencing, and development of a molecular serotyping scheme.
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DOI：
10.1371/journal.pone.0070329
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Reeves PR
中科院分区：
文献类型：
--
作者：
Hu D;Liu B;Dijkshoorn L;Wang L;Reeves PR
我们对20世纪90年代开发的鲍曼不动杆菌血清分型方案的模式菌株的基因簇进行了测序，并使用这些序列更好地了解了该属表面多糖的多样性。我们获得了27株现有血清型菌株的基因组序列，并鉴定了25个多糖基因簇序列。这些多糖中有12种都有结构，一般来说，目前存在的基因与已知的结构相适应。这极大地方便了解释。我们还发现了53个不同的糖基转移酶基因，其中7个菌株可以暂时将特定的基因分配给所有的连锁。我们确定了可以通过聚合酶链式反应或微阵列来区分这25种序列形式的引物，或者可以通过“分子血清学”来确定这些基因的血清型。我们将后者应用于190个不动杆菌基因组衍生基因簇，发现其中76个具有25种基因簇形式中的一种。我们还发现了新的基因簇，增加了52个不同wzy基因和不同基因含量的新基因簇序列形式。其中包括98株鲍曼不动杆菌(其中24株来自我们的菌株)和5株诺氏假丝酵母菌(3株来自我们的菌株)，而除鲍曼不动杆菌和诺氏假丝酵母菌以外的12个种的32个基因组都有新的序列形式。25个血清型序列中有1个在欧洲克隆I(EC I)中，2个在EC II中，而EC III中没有。公共基因组株增加了52个新的序列形式，使EC I中发现的数量达到5个，EC II中发现的数量达到9个，EC III中发现的数量达到2个。
We have sequenced the gene clusters for type strains of the Acinetobacter baumannii serotyping scheme developed in the 1990s, and used the sequences to better understand diversity in surface polysaccharides of the genus. We obtained genome sequences for 27 available serovar type strains, and identified 25 polysaccharide gene cluster sequences. There are structures for 12 of these polysaccharides, and in general the genes present are appropriate to the structure where known. This greatly facilitates interpretation. We also find 53 different glycosyltransferase genes, and for 7 strains can provisionally allocate specific genes to all linkages. We identified primers that will distinguish the 25 sequence forms by PCR or microarray, or alternatively the genes can be used to determine serotype by “molecular serology”. We applied the latter to 190 Acinetobacter genome-derived gene-clusters, and found 76 that have one of the 25 gene-cluster forms. We also found novel gene clusters and added 52 new gene-cluster sequence forms with different wzy genes and different gene contents. Altogether, the strains that have one of the original 25 sequence forms include 98 A. baumannii (24 from our strains) and 5 A. nosocomialis (3 from our strains), whereas 32 genomes from 12 species other than A. baumannii or A. nosocomialis, all have new sequence forms. One of the 25 serovar type sequences is found to be in European clone I (EC I), 2 are in EC II but none in EC III. The public genome strains add an additional 52 new sequence forms, and also bring the number found in EC I to 5, in EC II to 9 and in EC III to 2.
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影响因子：
3.1
作者：
Fregolino, Eleonora;Gargiulo, Valentina;De Castro, Cristina
通讯作者：
De Castro, Cristina
影响因子：
4.3
作者：
Cunneen, Monica M.;Pacinelli, Elvia;Reeves, Peter R.
通讯作者：
Reeves, Peter R.
影响因子：
4.2
作者：
Di Nocera PP;Rocco F;Giannouli M;Triassi M;Zarrilli R
通讯作者：
Zarrilli R
影响因子：
2.8
作者：
Hanuszkiewicz, Anna;Kaczynski, Zhigniew;Holst, Otto
通讯作者：
Holst, Otto
影响因子：
11.8
作者：
Chuang, Yu-Chung;Sheng, Wang-Huei;Chang, Shan-Chwen
通讯作者：
Chang, Shan-Chwen