Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats.
Alteration of cartilage surface collagen fibers differs locally after immobilization of knee joints in rats.
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DOI：
10.1111/joa.12290
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发表时间：
2015-05
影响因子：
2.4
通讯作者：
Kuroki H
中科院分区：
文献类型：
--
作者：
Nagai M;Aoyama T;Ito A;Tajino J;Iijima H;Yamaguchi S;Zhang X;Kuroki H
本研究的目的是在一种大鼠制动模型中，观察不同部位和不同时间段的表面胶原纤维的超微结构变化。雄性Wistar大鼠随机分为组织学组和大体及超微结构评估组。所有动物的左膝通过外固定架固定1、2、4、8或16 周(n = 5/时间点)。在四个特定区域(股骨和胫骨的接触和外围区域)和两个区域(浅层和深层)获取和评估了膝关节内侧中髁区的矢状面组织学切片。采用天狼星红染色对软骨中胶原纤维的染色强度进行半定量。用扫描电子显微镜观察各评价区域软骨表面的变化。从组织学和扫描电子显微镜观察，周围区软骨表面的原纤化和不规则变化比接触区更严重。有趣的是，在固定后16 周，我们观察到接触和周围区域的非纤维结构。接触区胶原纤维染色强度较周边区减弱。综上所述，固定后不同软骨区域表面胶原纤维超微结构的改变及胶原染色强度不同。这些结果表明，软骨的进行性退变是区域性的，并依赖于固定期的长度。
The purpose of this study was to examine the ultrastructural changes of surface cartilage collagen fibers, which differ by region and the length of the experimental period in an immobilization model of rat. Male Wistar rats were randomly divided into histological or macroscopic and ultrastructural assessment groups. The left knees of all the animals were surgically immobilized by external fixation for 1, 2, 4, 8 or 16 weeks (n = 5/time point). Sagittal histological sections of the medial mid-condylar region of the knee were obtained and assessed in four specific regions (contact and peripheral regions of the femur and tibia) and two zones (superficial and deep). To semi-quantify the staining intensity of the collagen fibers in the cartilage, picrosirius red staining was used. The cartilage surface changes of all the assessed regions were investigated by scanning electron microscopy (SEM). From histological and SEM observations, the fibrillation and irregular changes of the cartilage surface were more severe in the peripheral region than in the contact region. Interestingly, at 16 weeks post-immobilization, we observed non-fibrous structures at both the contact and peripheral regions. The collagen fiber staining intensity decreased in the contact region compared with the peripheral region. In conclusion, the alteration of surface collagen fiber ultrastructure and collagen staining intensity differed by the specific cartilage regions after immobilization. These results demonstrate that the progressive degeneration of cartilage is region specific, and depends on the length of the immobilization period.
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影响因子：
2.8
作者：
BEHRENS, F;KRAFT, EL;OEGEMA, TR
通讯作者：
OEGEMA, TR
影响因子：
27.4
作者：
LEDINGHAM, J;REGAN, M;DOHERTY, M
通讯作者：
DOHERTY, M
影响因子：
7
作者：
Moriyama, H.;Yoshimura, O.;Tobimatsu, Y.
通讯作者：
Tobimatsu, Y.
影响因子：
27.4
作者：
Haapala, J;Arokoski, JPA;Kiviranta, I
通讯作者：
Kiviranta, I
影响因子：
2.4
作者：
Julkunen, Petro;Halmesmaki, Esa P.;Helminen, Heikki J.
通讯作者：
Helminen, Heikki J.