The deletion of Math5 disrupts retinal blood vessel and glial development in mice.
The deletion of Math5 disrupts retinal blood vessel and glial development in mice.
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DOI：
10.1016/j.exer.2011.12.005
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发表时间：
2012-03
影响因子：
3.4
通讯作者：
Lutty, Gerard A.
中科院分区：
文献类型：
--
作者：
Edwards, Malia M.;McLeod, D. Scott;Li, Renzhong;Grebe, Rhonda;Bhutto, Imran;Mu, Xiuqian;Lutty, Gerard A.
视网膜血管的发育是一个尚未完全了解的复杂过程。该领域的大部分研究都集中在星形胶质细胞及其在视网膜内形成的模板上，这先于小鼠视网膜中的内皮细胞。然而，在人类和狗身上，星形胶质细胞的迁移紧随血管的发育，这表明其他类型的细胞可能指导这一过程。其中一种细胞类型是神经节细胞，它在血管形成前分化，位于初级视网膜血管丛附近。本研究利用Math5−/−小鼠研究了神经节细胞在血管发育中的潜在作用。先前有报道称Math5调节神经节细胞的分化，Math5−/−小鼠的这些细胞减少了95%。分别用分离素B4标记法和GFAP免疫组化法观察大鼠血管和神经胶质的发育情况。JB-4分析表明，玻璃状血管起源于视神经区附近的绒毛膜玻璃体血管。如前所述，Math5−/−小鼠具有基本的视神经。Math5−/−小鼠的初级视网膜血管在出生后没有发育，然而，玻璃状血管的分支最终进入视网膜并形成视网膜内毛细血管网络。星形胶质细胞模板仅在Math5−/−视网膜的某些区域形成。此外，GFAP+ myller细胞遍布视网膜，其长突包裹着玻璃状血管。透射电镜证实了<s:1>勒细胞异常，并显示了内限制膜的破坏。目前的数据表明，Math5−/−小鼠神经节细胞的丧失与视网膜血管发育的缺乏有关。
Retinal vascular development is a complex process that is not yet fully understood. The majority of research in this area has focused on astrocytes and the template they form in the inner retina, which precedes endothelial cells in the mouse retina. In humans and dogs, however, astrocyte migration follows behind development of blood vessels, suggesting that other cell types may guide this process. One such cell type is the ganglion cell, which differentiates before blood vessel formation and lies adjacent to the primary retinal vascular plexus. The present study investigated the potential role played by ganglion cells in vascular development using Math5−/− mice. It has previously been reported that Math5 regulates the differentiation of ganglion cells and Math5−/− mice have a 95% reduction in these cells. The development of blood vessels and glia was investigated using Griffonia simplicifolia isolectin B4 labeling and GFAP immunohistochemistry, respectively. JB-4 analysis demonstrated that the hyaloid vessels arose from choriovitreal vessels adjacent to the optic nerve area. As previously reported, Math5−/− mice had a rudimentary optic nerve. The primary retinal vessels did not develop post-natally in the Math5−/− mice, however, branches of the hyaloid vasculature eventually dove into the retina and formed the inner retinal capillary networks. An astrocyte template only formed in some areas of the Math5−/− retina. In addition, GFAP+ Müller cells were seen throughout the retina that had long processes wrapped around the hyaloid vessels. Transmission electron microscopy confirmed Müller cell abnormalities and revealed disruptions in the inner limiting membrane. The present data demonstrates that the loss of ganglion cells in the Math5−/− mice is associated with a lack of retinal vascular development.
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影响因子：
64.5
作者：
Mukouyama, Y;Shin, D;Anderson, DJ
通讯作者：
Anderson, DJ
影响因子：
4.4
作者：
Chan-Ling, T;McLeod, DS;Lutty, GA
通讯作者：
Lutty, GA
影响因子：
4.4
作者：
Baba, Takayuki;Grebe, Rhonda;Lutty, Gerard A.
通讯作者：
Lutty, Gerard A.
影响因子：
10.5
作者：
Wang, SW;Kim, BS;Gan, L
通讯作者：
Gan, L
影响因子：
25
作者：
Ghiasvand, Noor M.;Rudolph, Dellaney D.;Mashayekhi, Mohammad;Brzezinski, Joseph A.;Goldman, Daniel;Glaser, Tom
通讯作者：
Glaser, Tom