Morphological and Physiological Framework Underlying Plant Longevity in Arabidopsis thaliana.
Morphological and Physiological Framework Underlying Plant Longevity in Arabidopsis thaliana.
复制标题
拟南芥长寿的形态和生理结构。
DOI：
10.3389/fpls.2020.600726
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发表时间：
2020
影响因子：
5.6
通讯作者：
Ito T
中科院分区：
文献类型：
--
作者：
Wang Y;Kumaishi K;Suzuki T;Ichihashi Y;Yamaguchi N;Shirakawa M;Ito T
单核植物只有一个生殖阶段，它们的寿命是由分子网络发育决定的。在拟南芥（Arabidopsis thaliana）的生殖阶段，花序分生组织（IM）维持着一个干细胞的中心库，并产生有限数量的花原基，从而导致种子的形成和整个植物的死亡。在这项研究中，我们在细胞和细胞内的分辨率下观察到IM的形态学变化，直到植物生命周期结束。我们观察了从抽条后1周（WAB）到干细胞死亡期间的四个生物学事件：(1)IM的大小逐渐减小，(2)IM细胞的动态空泡化，(3)干细胞决定因子WUSCHEL （WUS）的表达缺失，(4)与干细胞死亡相关的程序性细胞死亡标志物双功能性核酶e1 （BFN1）的上调。这些结果表明，干细胞群在植物衰老过程中逐渐减少，最终完全终止。我们进一步发现，在5 WAB CLAVATA3 （CLV3）表达缺失之前，在IM中，WUS的表达在3 WAB时无法检测到；CLV3是WUS的负调节因子。此外，clv3植株的WUS损失延迟，寿命比野生型植株长6周。这些结果表明，CLV3在4-5个WAB上的延长表达可能是抑制WUS再激活和促进植物死亡的一种保护措施。最后，通过转录组分析，我们确定活性氧（ROS）参与了植物寿命的控制。我们的研究提出了拟南芥植物寿命调控的形态学和生理学框架。
Monocarpic plants have a single reproductive phase, in which their longevity is developmentally programmed by molecular networks. In the reproductive phase of Arabidopsis thaliana, the inflorescence meristem (IM) maintains a central pool of stem cells and produces a limited number of flower primordia, which result in seed formation and the death of the whole plant. In this study, we observed morphological changes in the IM at cellular and intracellular resolutions until the end of the plant life cycle. We observed four biological events during the periods from 1 week after bolting (WAB) till the death of stem cells: (1) the gradual reduction in the size of the IM, (2) the dynamic vacuolation of IM cells, (3) the loss of the expression of the stem cell determinant WUSCHEL (WUS), and (4) the upregulation of the programmed cell death marker BIFUNCTIONAL NUCLEASE1 (BFN1) in association with the death of stem cells. These results indicate that the stem cell population gradually decreases in IM during plant aging and eventually is fully terminated. We further show that the expression of WUS became undetectable in IM at 3 WAB prior to the loss of CLAVATA3 (CLV3) expression at 5 WAB; CLV3 is a negative regulator of WUS. Moreover, clv3 plants showed delayed loss of WUS and lived 6 weeks longer compared with wild-type plants. These results indicated that the prolonged expression of CLV3 at 4–5 WAB may be a safeguard that inhibits the reactivation of WUS and promotes plant death. Finally, through transcriptome analysis, we determined that reactive oxygen species (ROS) are involved in the control of plant longevity. Our work presents a morphological and physiological framework for the regulation of plant longevity in Arabidopsis.
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通讯作者：
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