Human Coronavirus NL63 Molecular Epidemiology and Evolutionary Patterns in Rural Coastal Kenya.
Human Coronavirus NL63 Molecular Epidemiology and Evolutionary Patterns in Rural Coastal Kenya.
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DOI：
10.1093/infdis/jiy098
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发表时间：
2018-05-05
期刊：
影响因子：
--
通讯作者：
Cotten M
中科院分区：
文献类型：
--
作者：
Kiyuka PK;Agoti CN;Munywoki PK;Njeru R;Bett A;Otieno JR;Otieno GP;Kamau E;Clark TG;van der Hoek L;Kellam P;Nokes DJ;Cotten M
人类冠状病毒 NL63 (HCoV-NL63) 是一种全球流行的病原体，可引起轻度和重度呼吸道感染，并在一生中反复发生再感染。通过基于社区和医院的监测在肯尼亚沿海收集了鼻腔样本。通过多重实时逆转录 PCR 检测 HCoV-NL63，并对阳性样本进行刺突 (S) 蛋白的核苷酸测序。此外，还选择了 25 名具有重复 HCoV-NL63 感染证据的个体的配对样本进行全基因组病毒测序。 1.3% (75/5573) 入院的肺炎儿童中检测到 HCoV-NL63。 2008 年至 2014 年间，两种 HCoV-NL63 基因型在基利菲传播。全基因组序列形成了一个单系进化枝，与来自全球其他地区的当代 HCoV-NL63 密切相关。观察到一种意想不到的重复感染模式，一些人在第二次感染期间表现出更高的病毒滴度。其他 2 种地方性冠状病毒 HCoV-229E 和 HCoV-OC43 也观察到类似的模式。 HCoV-NL63 的重复感染不伴有可检测的基因型转换。在肯尼亚沿海地区，HCoV-NL63 在住院儿童肺炎患者中表现出较低的患病率。遗传多样性低的进化枝持久性表明免疫选择有限，并且在再感染中没有可检测到的进化枝转换表明初始暴露不足以引发保护性免疫反应。人类冠状病毒 NL63 感染很常见，并且在一生中会反复发生再感染。重复感染的一部分显示病毒复制增强，但没有基因型转换的证据，表明初始暴露不足以引发保护性免疫反应。
Human coronavirus NL63 (HCoV-NL63) is a globally endemic pathogen causing mild and severe respiratory tract infections with reinfections occurring repeatedly throughout a lifetime. Nasal samples were collected in coastal Kenya through community-based and hospital-based surveillance. HCoV-NL63 was detected with multiplex real-time reverse transcription PCR, and positive samples were targeted for nucleotide sequencing of the spike (S) protein. Additionally, paired samples from 25 individuals with evidence of repeat HCoV-NL63 infection were selected for whole-genome virus sequencing. HCoV-NL63 was detected in 1.3% (75/5573) of child pneumonia admissions. Two HCoV-NL63 genotypes circulated in Kilifi between 2008 and 2014. Full genome sequences formed a monophyletic clade closely related to contemporary HCoV-NL63 from other global locations. An unexpected pattern of repeat infections was observed with some individuals showing higher viral titers during their second infection. Similar patterns for 2 other endemic coronaviruses, HCoV-229E and HCoV-OC43, were observed. Repeat infections by HCoV-NL63 were not accompanied by detectable genotype switching. In this coastal Kenya setting, HCoV-NL63 exhibited low prevalence in hospital pediatric pneumonia admissions. Clade persistence with low genetic diversity suggest limited immune selection, and absence of detectable clade switching in reinfections indicates initial exposure was insufficient to elicit a protective immune response. Infections with human coronavirus NL63 are common and reinfections occur repeatedly throughout life. A subset of repeat infections show enhanced virus replication with no evidence of genotype switching, indicating that initial exposure is insufficient to elicit a protective immune response.
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DOI：
10.1093/infdis/jis570
发表时间：
2012-11-15
期刊：
The Journal of infectious diseases
影响因子：
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作者：
Agoti CN;Mwihuri AG;Sande CJ;Onyango CO;Medley GF;Cane PA;Nokes DJ
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DOI：
10.1016/s0140-6736(03)13077-2
发表时间：
2003-04-19
期刊：
Lancet (London, England)
影响因子：
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期刊：
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影响因子：
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