From the animal house to the field: Are there consistent individual differences in immunological profile in wild populations of field voles (Microtus agrestis)?
From the animal house to the field: Are there consistent individual differences in immunological profile in wild populations of field voles (Microtus agrestis)?
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DOI：
10.1371/journal.pone.0183450
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Begon M
中科院分区：
文献类型：
--
作者：
Arriero E;Wanelik KM;Birtles RJ;Bradley JE;Jackson JA;Paterson S;Begon M
生活在远离自然的简单实验室环境中的近交系小鼠，其免疫反应表现出一致的变化。然而，野生动物种群通常是远系繁殖的，面临着寄生虫学和其他方面的多重挑战。在这项研究中，我们寻求证据的一致性差异，在野外的个人之间的免疫概况。在这种情况下，我们应用一种新的方法，使用纵向（重复捕获）的数据，从自然种群的田鼠，田鼠，在一系列的生活史和感染指标，基因表达水平。我们关注三个免疫基因，IFN-γ，Gata 3和IL-10，分别代表Th 1，Th 2和免疫应答的调节元件。我们的研究结果表明，有明确的证据表明，在考虑了其他测量的变异来源后，个体之间至少有一个免疫基因的典型表达水平存在一致差异，最多是所有三个免疫基因。此外，通过增加Gata 3表达水平来响应变化的环境的个体具有IFN-γ表达水平的相关增加。我们的工作强调了在野生动物种群中寄生虫学和传染病风险研究中承认个体之间免疫变异的重要性。
Inbred mouse strains, living in simple laboratory environments far removed from nature, have been shown to vary consistently in their immune response. However, wildlife populations are typically outbreeding and face a multiplicity of challenges, parasitological and otherwise. In this study we seek evidence of consistent difference in immunological profile amongst individuals in the wild. We apply a novel method in this context, using longitudinal (repeated capture) data from natural populations of field voles, Microtus agrestis, on a range of life history and infection metrics, and on gene expression levels. We focus on three immune genes, IFN-γ, Gata3, and IL-10, representing respectively the Th1, Th2 and regulatory elements of the immune response. Our results show that there was clear evidence of consistent differences between individuals in their typical level of expression of at least one immune gene, and at most all three immune genes, after other measured sources of variation had been taken into account. Furthermore, individuals that responded to changing circumstances by increasing expression levels of Gata3 had a correlated increase in expression levels of IFN-γ. Our work stresses the importance of acknowledging immunological variation amongst individuals in studies of parasitological and infectious disease risk in wildlife populations.
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