Identification and complete genome sequence of iris potyvirus A, which causes dwarfing and foliar chlorosis with mosaic or mottle disease symptoms on lily (Lilium lancifolium Thunb.) in China.
Identification and complete genome sequence of iris potyvirus A, which causes dwarfing and foliar chlorosis with mosaic or mottle disease symptoms on lily (Lilium lancifolium Thunb.) in China.
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DOI：
10.1016/j.virusres.2023.199141
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发表时间：
2023-09
期刊：
影响因子：
5
通讯作者：
Li, Cheng
中科院分区：
文献类型：
--
作者：
Wang, Fang;Yan, Dankan;Han, Kelei;Gao, Zhengliang;Ma, Chao;Chen, Ying;Bao, Xianxun;Li, Cheng
IrPVA-安徽天然侵染百合植物在中国的首次报道。 IrPVA-安徽是马铃薯病毒科马铃薯病毒属的新成员。它可以感染原寄主百合回接种的健康植株。在中国安徽省发现了百合植物（Lilium lancifolium Thunb.），表现出矮化和叶黄化，并伴有花叶病或斑驳病症状。我们利用小RNA高通量测序技术对安徽省百合种植区的病毒进行了调查。在这里，我们报告了病毒因子的鉴定和完整基因组序列。它含有 9733 个核苷酸（不包括聚腺苷酸尾），并编码 3063 个氨基酸的多蛋白。完整的多蛋白 ORF 与虹膜马铃薯Y病毒 A (GenBank MH898493) 的氨基酸序列同一性为 98.92%。外壳蛋白序列的系统发育分析表明，该病毒与马铃薯病毒科马铃薯Y病毒属的成员很接近，因此暂时命名为虹膜马铃薯Y病毒A安徽分离株（IrPVA-安徽）。这是百合植物中第一个完整的IrPVA-Anhui基因组序列，此前仅报道了来自Iris Domestica的部分序列。将该基因组序列与密切相关的马铃薯Y病毒组的基因组序列进行比较分析，确定了马铃薯Y病毒组典型的九个切割位点和保守基序。随后使用血清学测定（ELISA 和基于抗体的侧流测定）、分子方法 (RT-PCR) 和致病性测试进行病毒鉴定。通过透射电子显微镜（TEM）观察到长度约为700 nm的病毒颗粒，与马铃薯Y病毒属的病毒相似。我们对多个物种的健康植物进行回接种，以调查病毒的宿主范围。它感染原始宿主鸢尾花和本塞姆氏烟草，但不感染普通小麦、豌豆、苋菜藜或曼陀罗。这是我国首次报道百合植株自然感染IrPVA-Anhui，为今后百合种植中IrPVA-Anhui的防治提供科学依据。
First report of IrPVA-Anhui natural infect lily plants in China. IrPVA-Anhui is a new member of the genus Potyvirus in the family Potyviridae. It can infect the original host lily back-inoculated healthy plants. Lily plants (Lilium lancifolium Thunb.) exhibiting dwarfing and foliar chlorosis with mosaic or mottle disease symptoms were found in Anhui Province, China. We used high-throughput sequencing of small RNA to survey the virus in the lily cultivation region of Anhui Province. Here, we report the identification and complete genome sequence of the viral agent. It contains 9733 nucleotides, excluding the poly(A) tail, and encodes a polyprotein of 3063 amino acids. The complete polyprotein ORF shows 98.92% amino acid sequence identity with that of iris potyvirus A (GenBank MH898493). Phylogenetic analysis of coat protein sequences placed the viral agent close to members of the genus Potyvirus in the family Potyviridae, and it was therefore provisionally named iris potyvirus A isolate Anhui (IrPVA-Anhui). This is the first complete genome sequence of IrPVA-Anhui from lily plant, for which only a partial sequence from Iris domestica has been reported previously. Comparative analysis of this genome sequence with those of closely related potyviruses identified nine cleavage sites and the conserved motifs typical of potyviruses. Subsequent virus identification was performed using serological assays (ELISA and antibody-based lateral flow assays), molecular methods (RT-PCR), and a pathogenicity test. Virus particles with a length of about 700 nm, similar to viruses in the genus Potyvirus, were observed via transmission electron microscope (TEM). We back-inoculated healthy plants of multiple species to investigate the host range of the virus. It infected the original host, Iris domestica, and Nicotiana benthamiana but not Triticum aestivum, Pisum sativum, Chenopodium amaranticolor, or Datura stramonium. This is the first report of natural IrPVA-Anhui infection of lily plants in China, providing a scientific basis for IrPVA-Anhui control in future lily plantings.
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影响因子：
3.1
作者：
ROBINSON, DJ
通讯作者：
ROBINSON, DJ
影响因子：
2.7
作者：
Komatsu, K.;Yamaji, Y.;Namba, S.
通讯作者：
Namba, S.
影响因子：
3.7
作者：
DOUGHERTY, WG;PARKS, TD;FLETTERICK, RJ
通讯作者：
FLETTERICK, RJ
巴黎病毒1号全基因组序列分析：感染重楼的马铃薯蒂病毒属新成员。
影响因子：
2.7
作者：
Chen, Lu;Anane, Rex Frimpong;Zhao, Mingfu
通讯作者：
Zhao, Mingfu
DOI：
10.1073/pnas.90.24.11919
发表时间：
1993-12-15
影响因子：
11.1
作者：
ATREYA, CD;PIRONE, TP
通讯作者：
PIRONE, TP