Immunohistochemical detection of connexin36 in sympathetic preganglionic and somatic motoneurons in the adult rat.
Immunohistochemical detection of connexin36 in sympathetic preganglionic and somatic motoneurons in the adult rat.
复制标题
成年大鼠的交感神经前和体细胞神经元中连接性的连接蛋白36的免疫组织化学检测。
DOI：
10.1016/j.autneu.2007.12.004
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发表时间：
2008-05-30
影响因子：
2.7
通讯作者：
Gilbey, Michael P.
中科院分区：
文献类型：
--
作者：
Marina, Nephtali;Becker, David L.;Gilbey, Michael P.
成年人中枢神经系统的缝隙连接通讯在神经元活动的同步化中起着重要作用。体外研究表明，在新生大鼠和成年大鼠脊髓中，交感神经节前运动神经元之间以及躯体运动神经元之间的缝隙连接存在电紧张性耦合。膜振荡的电紧张性传递可能是神经元募集的重要机制，并导致群体水平上有节律的交感神经和躯体运动活动的产生。成人脊髓中的缝隙连接主要由连接蛋白36（Cx 36）构成。然而，Cx 36在脊髓特定神经元群体中的分布尚不清楚。在这里，我们确定Cx 36样免疫反应在交感神经节前和躯体运动神经元在胸段的成年大鼠脊髓。为此，对取自T6-T8脊髓节段的横切面（20 μm）进行针对Cx 36和胆碱乙酰转移酶（ChAT）的双重免疫染色。Cx 36点状免疫染色检测到大多数的ChAT免疫反应（IR）神经元从第七层[中间外侧细胞柱（IML）和闰细胞组（IC）]，第X层[中央自主神经核（CA）]和腹角神经元从第八层和第九层。Cx 36点分布于神经元胞体内和沿着树突突起。Cx 36在ChAT-IR神经元的存在是一致的交感神经节前运动神经元之间的电耦合和躯体运动神经元之间通过缝隙连接在成人脊髓。
Gap junctional communication in the adult CNS plays an important role in the synchronization of neuronal activities. In vitro studies have shown evidence of electrotonic coupling through gap junctions between sympathetic preganglionic motoneurons and between somatic motoneurons in the neonatal and adult rat spinal cord. Electrotonic transmission of membrane oscillations might be an important mechanism for recruitment of neurons and result in the generation of rhythmic sympathetic and somato-motor activity at the population level. Gap junctions in the adult spinal cord are constituted principally by connexin36 (Cx36). However, the distribution of Cx36 in specific neuronal populations of the spinal cord is unknown. Here, we identify Cx36-like immunoreactivity in sympathetic preganglionic and somatic motoneurons in thoracic spinal cord segments of the adult rat. For this purpose, double immunostaining against Cx36 and choline acetyltransferase (ChAT) was performed on transverse sections (20 μm) taken from spinal segments T6–T8. Cx36 punctate immunostaining was detected in the majority of ChAT-immunoreactive (-ir) neurons from lamina VII [intermediolateral cell column (IML) and intercalated cell group (IC)], lamina X [central autonomic nucleus (CA)] and in ventral horn neurons from laminae VIII and IX. Cx36 puncta were distributed in the neuronal somata and along dendritic processes. The presence of Cx36 in ChAT-ir neurons is consistent with electrical coupling between sympathetic preganglionic motoneurons and between somatic motoneurons through gap junctions in the adult spinal cord.
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影响因子：
2.5
作者：
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通讯作者：
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