Characterization of chicken secretin (SCT) and secretin receptor (SCTR) genes: A novel secretin-like peptide (SCT-LP) and secretin encoded in a single gene
Characterization of chicken secretin (SCT) and secretin receptor (SCTR) genes: A novel secretin-like peptide (SCT-LP) and secretin encoded in a single gene
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鸡促胰液素 (SCT) 和促胰液素受体 (SCTR) 基因的表征：一种新型促胰液素样肽 (SCT-LP) 和单基因编码的促胰液素
DOI：
10.1016/j.mce.2011.09.012
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发表时间：
2012-01
影响因子：
4.1
通讯作者：
Leung, Frederick C.
中科院分区：
文献类型：
--
作者：
Wang, Yajun;Huang, Guian;Li, Juan;Meng, Fengyan;He, Xiaping;Leung, Frederick C.
促胰液素和促胰液素受体在调节哺乳动物胰腺水和碳酸氢盐分泌中起重要作用，然而，对它们在包括鸟类在内的非哺乳类脊椎动物中的表达、结构和生物学功能知之甚少。本研究首次从成年鸡的十二指肠中克隆了编码分泌素和分泌素受体的全长cDNA。假定的鸡促分泌素受体（cSCTR）长度为449个氨基酸，并且与其哺乳动物对应物具有高序列同一性（58-63%）。有趣的是，鸡分泌素cDNA不仅编码分泌素肽（cSCT），而且编码一种新的分泌素样肽（cSCT-LP），其与鸡（56%）和哺乳动物（48-52%）的分泌素具有高度的氨基酸同源性。使用pGL 3-CRE-荧光素酶报告系统，我们进一步证明了cSCT（EC 50：0.31nM）和cSCT-LP（EC 50：1.10nM），而不是其他结构相关的肽，可以有效地激活CHO细胞中表达的cSCTR，这表明这两种肽可以作为cSCTR的潜在配体。利用RT-PCR技术检测了成年鸡组织中促胰液素及其受体的表达。促分泌素主要在小肠中表达，而cSCTR的mRNA表达仅限于几种组织，包括胃肠道、肝脏、睾丸、胰腺和几个脑区域。总之，本研究结果不仅为阐明SCT、SCT-LP和SCTR在鸡中的生理作用奠定了分子基础，而且为研究脊椎动物进化过程中分泌素及其受体的结构和功能变化提供了重要见解。
Secretin and the secretin receptor have been reported to play an important role in regulating pancreatic water and bicarbonate secretion in mammals; however, little is known about their expression, structure, and biological functions in non-mammalian vertebrates including birds. In this study, the full-length cDNAs encoding secretin and secretin receptor have first been cloned from duodenum of adult chickens. The putative chicken secretin receptor (cSCTR) is 449 amino acids in length and shares high sequence identity (58–63%) with its mammalian counterparts. Interestingly, chicken secretin cDNA encodes not only the secretin peptide (cSCT), but also a novel secretin-like peptide (cSCT-LP), which shares high amino acid identity with chicken (56%) and mammalian (48–52%) secretin. Using a pGL3-CRE-luciferase reporter system, we further demonstrated that both cSCT (EC50: 0.31nM) and cSCT-LP (EC50: 1.10nM), but not other structurally-related peptides, could potently activate cSCTR expressed in CHO cells, suggesting that both peptides may function as potential ligands for cSCTR. Using RT-PCR, the expression of secretin and secretin receptor in adult chicken tissues was also examined. Secretin was detected to be predominantly expressed in small intestine, while the mRNA expression of cSCTR was restricted to several tissues including gastrointestinal tract, liver, testis, pancreas and several brain regions. Collectively, results from present study not only established a molecular basis to elucidate the physiological roles of SCT, SCT-LP and SCTR in chickens, but also provide critical insights into structural and functional changes of secretin and its receptor during vertebrate evolution.
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影响因子：
3.7
作者：
Tam JK;Lau KW;Lee LT;Chu JY;Ng KM;Fournier A;Vaudry H;Chow BK
通讯作者：
Chow BK
影响因子：
3.4
作者：
Cardoso JC;Vieira FA;Gomes AS;Power DM
通讯作者：
Power DM
影响因子：
2.7
作者：
G. Dockray
通讯作者：
G. Dockray
影响因子：
5
作者：
L. Angelucci;M. Baldieri;G. Linari
通讯作者：
L. Angelucci;M. Baldieri;G. Linari
DOI：
10.1095/biolreprod.104.028399
发表时间：
2004-09
期刊：
--
影响因子：
--
作者：
Yajun Wang;W. Ge
通讯作者：
Yajun Wang;W. Ge