Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration.
Biomimetic Design and Fabrication of Sericin-Hydroxyapatite Based Membranes With Osteogenic Activity for Periodontal Tissue Regeneration.
复制标题
用于牙周组织再生的具有成骨活性的丝胶-羟基磷灰石基膜的仿生设计与制造
DOI：
10.3389/fbioe.2022.899293
复制
发表时间：
2022
影响因子：
5.7
通讯作者：
Tao, Gang
中科院分区：
文献类型：
--
作者：
Ming, Piaoye;Rao, Pengcheng;Wu, Tianli;Yang, Jianghua;Lu, Shi;Yang, Binbin;Xiao, Jingang;Tao, Gang
关键词：
引导组织再生技术是治疗牙周组织缺损的一种很有前途的方法。GTR膜构建机械屏障以控制牙龈上皮的生长，并为牙周组织，特别是牙槽骨的再生提供适当的空间。然而，现有的GTR膜只是作为屏障，缺乏诱导牙槽骨再生的生物活性。本研究以丝胶为有机模板，采用仿生矿化方法制备了丝胶-羟基磷灰石(Ser-HAP)复合纳米材料。矿化的丝氨酸-羟基磷灰石具有良好的生物相容性，可促进人牙周膜干细胞向成骨细胞分化。采用冻融法将Ser-HAP与PVA结合形成PVA/Ser-HAP复合膜。进一步的研究证实PVA/Ser-HAP膜不影响hPDLSCs的活性。此外，碱性磷酸酶(ALP)染色、茜素红染色(ARS)和RT-qPCR检测表明PVA/Ser-HAP膜通过激活成骨细胞相关基因ALP、Runx2、OCN和OPN的表达而诱导hPDLSCs成骨分化。基于丝氨酸-羟基磷灰石的独特的GTR膜在不添加任何诱导剂的情况下诱导hPDLSCs向成骨细胞分化，显示了良好的牙周再生治疗潜力。
The guided tissue regeneration (GTR) technique is a promising treatment for periodontal tissue defects. GTR membranes build a mechanical barrier to control the ingrowth of the gingival epithelium and provide appropriate space for the regeneration of periodontal tissues, particularly alveolar bone. However, the existing GTR membranes only serve as barriers and lack the biological activity to induce alveolar bone regeneration. In this study, sericin-hydroxyapatite (Ser-HAP) composite nanomaterials were fabricated using a biomimetic mineralization method with sericin as an organic template. The mineralized Ser-HAP showed excellent biocompatibility and promoted the osteogenic differentiation of human periodontal membrane stem cells (hPDLSCs). Ser-HAP was combined with PVA using the freeze/thaw method to form PVA/Ser-HAP membranes. Further studies confirmed that PVA/Ser-HAP membranes do not affect the viability of hPDLSCs. Moreover, alkaline phosphatase (ALP) staining, alizarin red staining (ARS), and RT-qPCR detection revealed that PVA/Ser-HAP membranes induce the osteogenic differentiation of hPDLSCs by activating the expression of osteoblast-related genes, including ALP, Runx2, OCN, and OPN. The unique GTR membrane based on Ser-HAP induces the differentiation of hPDLSCs into osteoblasts without additional inducers, demonstrating the excellent potential for periodontal regeneration therapy.
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生物相容性纳米复合材料增强牙周膜干细胞的成骨和牙骨质分化，促进牙周再生
DOI：
10.3390/ma13214951
发表时间：
2020-11-04
期刊：
Materials (Basel, Switzerland)
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影响因子：
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