Identification of FadT as a Novel Quorum Quenching Enzyme for the Degradation of Diffusible Signal Factor in Cupriavidus pinatubonensis Strain HN-2.
Identification of FadT as a Novel Quorum Quenching Enzyme for the Degradation of Diffusible Signal Factor in Cupriavidus pinatubonensis Strain HN-2.
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鉴定 FadT 作为一种新型群体淬灭酶，用于降解 Cupriavidus pinatubonensis 菌株 HN-2 中的扩散信号因子
DOI：
10.3390/ijms22189862
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发表时间：
2021-09-13
影响因子：
5.6
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Xu X;Ye T;Zhang W;Zhou T;Zhou X;Dai W;Chen S
群体感应（QS）是微生物细胞间的一种通讯机制，在细菌感染中起着重要作用。QS介导的细菌感染可以通过群体淬灭（QQ）来阻断，这阻碍了信号积累、识别和通信。许多细菌的致病性，包括野油菜黄单胞菌致病变种。campestris（Xcc）是一种受扩散信号因子（DSF）调控的脂肪酸信号分子。PinatubonensisHN-2通过降解DSF，可显著降低QQ对XCC的侵染。另一方面，菌株HN-2中的QQ机制尚不清楚。为了了解QQ在HN-2菌株中的分子机制，我们使用全基因组测序和比较基因组学研究。我们发现fadT基因编码酰基辅酶A脱氢酶作为一种新的QQ酶。定点突变的结果表明，HN-2菌株降解DSF需要fadT基因。纯化的FadT在宽pH和温度范围内表现出高酶活性和出色的稳定性，在pH 7.0和35 °C下具有最大活性。FadT酶活性不需要辅因子。该酶具有较强的降解DSF的能力。此外，fadT在Xcc中的表达导致在寄主植物中的致病性显著降低，如大白菜、萝卜和小白菜。综上所述，我们的研究结果确定了一种新的DSF降解酶，FadT，在C。pinatubonensis HN-2，这表明其在生物防治DSF介导的病原体中的潜在用途。
Quorum sensing (QS) is a microbial cell–cell communication mechanism and plays an important role in bacterial infections. QS-mediated bacterial infections can be blocked through quorum quenching (QQ), which hampers signal accumulation, recognition, and communication. The pathogenicity of numerous bacteria, including Xanthomonas campestris pv. campestris (Xcc), is regulated by diffusible signal factor (DSF), a well-known fatty acid signaling molecule of QS. Cupriavidus pinatubonensis HN-2 could substantially attenuate the infection of XCC through QQ by degrading DSF. The QQ mechanism in strain HN-2, on the other hand, is yet to be known. To understand the molecular mechanism of QQ in strain HN-2, we used whole-genome sequencing and comparative genomics studies. We discovered that the fadT gene encodes acyl-CoA dehydrogenase as a novel QQ enzyme. The results of site-directed mutagenesis demonstrated the requirement of fadT gene for DSF degradation in strain HN-2. Purified FadT exhibited high enzymatic activity and outstanding stability over a broad pH and temperature range with maximal activity at pH 7.0 and 35 °C. No cofactors were required for FadT enzyme activity. The enzyme showed a strong ability to degrade DSF. Furthermore, the expression of fadT in Xcc results in a significant reduction in the pathogenicity in host plants, such as Chinese cabbage, radish, and pakchoi. Taken together, our results identified a novel DSF-degrading enzyme, FadT, in C. pinatubonensis HN-2, which suggests its potential use in the biological control of DSF-mediated pathogens.
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影响因子：
11.3
作者：
An SQ;Potnis N;Dow M;Vorhölter FJ;He YQ;Becker A;Teper D;Li Y;Wang N;Bleris L;Tang JL
通讯作者：
Tang JL
影响因子：
2.7
作者：
Caicedo, J. C.;Villamizar, S.;Ferro, J. A.
通讯作者：
Ferro, J. A.
影响因子：
14.9
作者：
Eichinger V;Nussbaumer T;Platzer A;Jehl MA;Arnold R;Rattei T
通讯作者：
Rattei T
影响因子：
14.9
作者：
Bairoch, A;Apweiler, R
通讯作者：
Apweiler, R
影响因子：
4.1
作者：
Fetzner, Susanne
通讯作者：
Fetzner, Susanne