Microfluidic diagnostic tool for the developing world: contactless impedance flow cytometry.

Microfluidic diagnostic tool for the developing world: contactless impedance flow cytometry.
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DOI:
10.1039/c2lc40759k
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发表时间:
2012-11-07
期刊:
影响因子:
6.1
通讯作者:
Davis RW
Davis RW
中科院分区:
工程技术1区
文献类型:
--
作者:
Emaminejad S;Javanmard M;Dutton RW;Davis RW

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In this work, we demonstrate a novel and cost-effective approach to implement a disposable microfluidic contactless impedance cytometer. Conventional methods for single cell impedance cytometry use microfabricated electrodes in direct contact with the buffer to measure changes of its electrical impedance when cells pass through the applied electric field. However, this approach requires expensive microfabrication of electrodes, and also, the fabricated electrodes cannot be reused without thorough and time-consuming cleaning process. Here, we introduce a novel approach to allow for single cell impedance cytometry using electrodes that can be reused, without the need for microfabrication of the electrodes. This disposable device can be potentially inserted onto a Printed Circuit board (PCB) which has a non-disposable, yet inexpensive, electronic reading apparatus. This significantly reduces the manufacturing costs, making it suitable for low resource settings, such as point-of-care testing in the developing countries.
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