Chronic rhinosinusitis with nasal polyps is characterized by B-cell inflammation and EBV-induced protein 2 expression.
Chronic rhinosinusitis with nasal polyps is characterized by B-cell inflammation and EBV-induced protein 2 expression.
复制标题
DOI：
10.1016/j.jaci.2013.01.043
复制
发表时间：
2013-04
影响因子：
14.2
通讯作者：
Schleimer, Robert P.
中科院分区：
文献类型：
--
作者：
Hulse, Kathryn E.;Norton, James E.;Suh, Lydia;Zhong, Qiu;Mahdavinia, Mahboobeh;Simon, Patrick;Kern, Robert C.;Conley, David B.;Chandra, Rakesh K.;Tan, Bruce K.;Peters, Anju T.;Grammer, Leslie C., III;Harris, Kathleen E.;Carter, Roderick G.;Kato, Atsushi;Schleimer, Robert P.
关键词：
尽管慢性鼻窦炎（CRS）的患病率和发病率很高，但对其发病机制知之甚少。最近的研究表明，B细胞可能在CRS中发挥重要作用。我们试图彻底表征CRS患者和健康对照受试者窦组织中的B谱系细胞，并确定CRS患者中EBV诱导蛋白2（已知在B细胞反应的发展中起重要作用）的水平是否增加。从CRS患者和健康对照受试者的窦组织中分离的细胞通过流式细胞术和免疫组织化学进行表征。通过使用多重微珠阵列和ELISA在组织提取物、鼻灌洗液和血清中测量抗体的局部产生。采用定量RT-PCR、ELISA和Western印迹法评估组织提取物的基因和蛋白表达。CRS患者的鼻息肉（NP）中B细胞和浆细胞水平均高于健康对照组钩突组织（P <0.05）。与正常钩突组织相比，NPs中的几种抗体同种型水平也显著升高（P <0.05），但循环抗体水平无差异。有趣的是，NPs中EBV诱导蛋白2的水平也升高（P <0.05），并与窦组织中浆细胞标志物（CD 138和B淋巴细胞诱导成熟蛋白）的表达呈正相关。B细胞和浆细胞富含NP，局部积极产生抗体，可能导致CRS患者的慢性炎症。阐明这种过度的局部B细胞反应的机制可能会为改进治疗策略的发展提供新的见解。
Despite the high prevalence and morbidity of chronic rhinosinusitis (CRS), little is known about the mechanisms that underlie its pathogenesis. Recent studies have suggested that B cells might play an important role in CRS. We sought to thoroughly characterize B lineage cells within sinus tissues of patients with CRS and healthy control subjects and to determine whether levels of EBV-induced protein 2, which is known to play an important role in the development of B-cell responses, were increased in patients with CRS. Cells isolated from sinus tissues of patients with CRS and healthy control subjects were characterized by means of flow cytometry and immunohistochemistry. Local production of antibodies was measured in tissue extracts, nasal lavage fluid, and sera by using multiplex bead arrays and ELISA. Quantitative RT-PCR, ELISA, and Western blotting were used to assess gene and protein expression from tissue extracts. Nasal polyps (NPs) from patients with CRS had increased levels of both B cells and plasma cells compared with uncinate tissue from healthy control subjects (P < .05). NPs also contained significantly increased levels of several antibody isotypes compared with normal uncinate tissue (P < .05), but no differences in circulating antibody levels were found. Interestingly, levels of EBV-induced protein 2 were also increased in NPs (P < .05) and were positively correlated with expression of plasma cell markers (CD138 and B lymphocyte–induced maturation protein) in sinus tissue. B cells and plasma cells are enriched in NPs, actively produce antibodies locally, and might contribute to chronic inflammation in patients with CRS. Elucidating the mechanisms that underlie this excessive local B-cell response might provide novel insights for the development of improved therapeutic strategies.
登录
查看更多内容
DOI：
10.1016/j.jaci.2012.02.046
发表时间：
2012-08
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Takabayashi T;Kato A;Peters AT;Suh LA;Carter R;Norton J;Grammer LC;Tan BK;Chandra RK;Conley DB;Kern RC;Fujieda S;Schleimer RP
通讯作者：
Schleimer RP
慢性鼻窦炎患者鼻息肉组织中抗菌 PLUNC 蛋白表达减少。
影响因子：
12.4
作者：
Seshadri S;Lin DC;Rosati M;Carter RG;Norton JE;Suh L;Kato A;Chandra RK;Harris KE;Chu HW;Peters AT;Tan BK;Conley DB;Grammer LC;Kern RC;Schleimer RP
通讯作者：
Schleimer RP
影响因子：
14.2
作者：
Dykewicz, Mark S.;Hamilos, Daniel L.
通讯作者：
Hamilos, Daniel L.
DOI：
10.4049/jimmunol.0801375
发表时间：
2009-06-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Kato A;Chustz RT;Ogasawara T;Kulka M;Saito H;Schleimer RP;Matsumoto K
通讯作者：
Matsumoto K
影响因子：
8
作者：
通讯作者：
--