Digitally programmable microfluidic automaton for multiscale combinatorial mixing and sample processing.
Digitally programmable microfluidic automaton for multiscale combinatorial mixing and sample processing.
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DOI：
10.1039/c2lc40861a
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发表时间：
2013-01-21
期刊：
影响因子：
6.1
通讯作者：
Mathies RA
中科院分区：
文献类型：
--
作者：
Jensen EC;Stockton AM;Chiesl TN;Kim J;Bera A;Mathies RA
由气动微阀的二维阵列组成的数字可编程微流体自动机经过编程可以执行新的多尺度混合和样品处理操作。通过在各个 nL 级阀门内精确计量多种试剂，然后连续重复转移到阵列中的编程位置，可以实现大（μL 级）体积处理操作。开发了一种利用新组合阀概念的新颖工艺，可在 800 毫秒内连续快速、完全混合试剂。混合、转移、储存和冲洗操作组合实施，以实现复杂的检测自动化方案。通过执行自动连续稀释进行定量分析以及首次在 Mars 有机分析仪上演示用于微芯片毛细管电泳的生物标志物目标（羧酸）的片上荧光衍生化，证明了该技术的实用性。开发了一种语言来描述如何组合单元操作以形成微流体程序。最后，该技术用于开发一种新型微流体 6 样品处理器，用于大组（> 2 个独特组合）试剂的组合混合。数字可编程微流体自动机是一种多功能可编程样品处理器，适用于各种处理体积、多个样品和不同类型的分析。
A digitally programmable microfluidic Automaton consisting of a 2-dimensional array of pneumatically actuated microvalves is programmed to perform new multiscale mixing and sample processing operations. Large (µL-scale) volume processing operations are enabled by precise metering of multiple reagents within individual nL-scale valves followed by serial repetitive transfer to programmed locations in the array. A novel process exploiting new combining valve concepts is developed for continuous rapid and complete mixing of reagents in less than 800 ms. Mixing, transfer, storage, and rinsing operations are implemented combinatorially to achieve complex assay automation protocols. The practical utility of this technology is demonstrated by performing automated serial dilution for quantitative analysis as well as the first demonstration of on-chip fluorescent derivatization of biomarker targets (carboxylic acids) for microchip capillary electrophoresis on the Mars Organic Analyzer. A language is developed to describe how unit operations are combined to form a microfluidic program. Finally, this technology is used to develop a novel microfluidic 6-sample processor for combinatorial mixing of large sets (>2 unique combinations) of reagents. The digitally programmable microfluidic Automaton is a versatile programmable sample processor for a wide range of process volumes, for multiple samples, and for different types of analyses.
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影响因子：
6.1
作者：
Goluch, Edgar D.;Nam, Jwa-Min;Liu, Chang
通讯作者：
Liu, Chang
影响因子：
6.1
作者：
Neils, C;Tyree, Z;Folch, A
通讯作者：
Folch, A
影响因子：
7.4
作者：
Liu, J;Hansen, C;Quake, SR
通讯作者：
Quake, SR
影响因子：
8.4
作者：
Grover, WH;Skelley, AM;Mathies, RA
通讯作者：
Mathies, RA
影响因子：
7.4
作者：
Chiesl, Thomas N.;Chu, Wai K.;Mathies, Richard A.
通讯作者：
Mathies, Richard A.