Microscopy with ultraviolet surface excitation (MUSE): A novel approach to real-time inexpensive slide-free dermatopathology.
Microscopy with ultraviolet surface excitation (MUSE): A novel approach to real-time inexpensive slide-free dermatopathology.
复制标题
带有紫外线表面激发的显微镜（MUSE）：一种新型的实时廉价幻灯片皮肤病理学的方法。
DOI：
10.1111/cup.13255
复制
发表时间：
2018-07
影响因子：
1.7
通讯作者：
Fung MA
中科院分区：
文献类型：
--
作者：
Qorbani A;Fereidouni F;Levenson R;Lahoubi SY;Harmany ZT;Todd A;Fung MA
传统的组织学依赖于处理和物理切片的冻结或形式上的石蜡包裹（FFPE）组织成薄的切片（通常为4-6μm），然后在标准的宽视野显微镜上进行染色和观看，并使用紫外线（UV）式兴奋的方法来进行替代式兴奋。来自切割表面的ality图像新鲜的或固定的组织在短暂的染色之后，没有固定，组织样本的嵌入和组织学切片。嗯，脊柱质，脊柱，脉络膜，胶原蛋白和弹性蛋白，汗腺，脂肪组织和炎性细胞以及4例基本细胞癌和1例pseudoxanthome弹性瘤的效果，这表明我们的组织范围很快，并且可以证明所有正常的构造，并且可以证明所有正常的特征，并且是众所周知的，这是一个正常的型号。和具有成本效益的诊断皮肤病理学方法。
Traditional histology relies on processing and physically sectioning either frozen or formalinfixed paraffin-embedded (FFPE) tissue into thin slices (typically 4–6 μm) prior to staining and viewing on a standard wide-field microscope. Microscopy using ultraviolet (UV) surface excitation (MUSE) represents a novel alternative microscopy method that works with UV excitation using oblique cis-illumination, which can generate high-quality images from the cut surface of fresh or fixed tissue after brief staining, with no requirement for fixation, embedding and histological sectioning of tissue specimens. We examined its potential utility in dermatopathology. Concordance between MUSE images and hematoxylin and eosin (H&E) slides was assessed by the scoring of MUSE images on their suitability for identifying 10 selected epidermal and dermal structures obtained from minimally fixed tissue, including stratum corneum, stratum granulosum, stratum spinosum, stratum basale, nerve, vasculature, collagen and elastin, sweat glands, adipose tissue and inflammatory cells, as well as 4 cases of basal cell carcinoma and 1 case of pseudoxanthoma elasticum deparaffinized out of histology blocks. Our results indicate that MUSE can identify nearly all normal skin structures seen on routine H&E as well as some histopathologic features, and appears promising as a fast, reliable and cost-effective diagnostic approach in dermatopathology.
登录
查看更多内容
影响因子：
2.5
作者：
Yew E;Rowlands C;So PT
通讯作者：
So PT
影响因子：
3.5
作者：
Meinhardt, Merve;Krebs, Ronald;Tronnier, Hagen
通讯作者：
Tronnier, Hagen
影响因子：
3.5
作者：
Lin, Bevin;Urayama, Shiro;Demos, Stavros G.
通讯作者：
Demos, Stavros G.
影响因子：
3.8
作者：
Lin, Bevin;Urayama, Shiro;Demos, Stavros G.
通讯作者：
Demos, Stavros G.
影响因子：
28.1
作者：
Fereidouni, Farzad;Harmany, Zachary T.;Levenson, Richard
通讯作者：
Levenson, Richard