The plasticity of the grapevine berry transcriptome.
The plasticity of the grapevine berry transcriptome.
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DOI：
10.1186/gb-2013-14-6-r54
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发表时间：
2013-06-07
期刊：
影响因子：
12.3
通讯作者：
Pezzotti M
中科院分区：
文献类型：
--
作者：
Dal Santo S;Tornielli GB;Zenoni S;Fasoli M;Farina L;Anesi A;Guzzo F;Delledonne M;Pezzotti M
表型可塑性是指一个单一的基因型可以表达作为其环境的功能表型的范围。这些表型变异归因于环境对影响塑料性状的基因的表达和功能的影响。我们研究了葡萄藤的表型可塑性，通过比较浆果转录组在一个单一的无性系的植物繁殖的常见葡萄藤品种葡萄属葡萄品种Corpus通过3个连续的生长年种植在11个不同的葡萄园在意大利维罗纳地区。大多数的浆果转录组聚类的生长年，而不是常见的环境条件或葡萄栽培的做法，和转录相关的次生代谢表现出对不同气候的高敏感性，也证实了从相同的样品中获得的代谢组学数据。当在1个生长年内对11个葡萄园进行分析时，对环境敏感的浆果转录组包含5%的蛋白质编码基因和18%的在浆果发育期间调节的转录本。可塑性基因在转录因子、翻译、运输和次生代谢等本体论类别中特别丰富。特定的塑料转录本与共享共同的葡萄栽培实践或环境条件的葡萄园群体相关，并且塑料转录组重编程在极端天气条件下更为强烈。我们还确定了一组缺乏可塑性的基因，在所有浆果中表现出组成型表达或类似的调节。我们的数据揭示了可能负责葡萄藤表型可塑性的候选基因，并为不同农业系统下葡萄藤转录组可塑性的表征迈出了第一步。
Phenotypic plasticity refers to the range of phenotypes a single genotype can express as a function of its environment. These phenotypic variations are attributable to the effect of the environment on the expression and function of genes influencing plastic traits. We investigated phenotypic plasticity in grapevine by comparing the berry transcriptome in a single clone of the vegetatively-propagated common grapevine species Vitis vinifera cultivar Corvina through 3 consecutive growth years cultivated in 11 different vineyards in the Verona area of Italy. Most of the berry transcriptome clustered by year of growth rather than common environmental conditions or viticulture practices, and transcripts related to secondary metabolism showed high sensitivity towards different climates, as confirmed also by metabolomic data obtained from the same samples. When analyzed in 11 vineyards during 1 growth year, the environmentally-sensitive berry transcriptome comprised 5% of protein-coding genes and 18% of the transcripts modulated during berry development. Plastic genes were particularly enriched in ontology categories such as transcription factors, translation, transport, and secondary metabolism. Specific plastic transcripts were associated with groups of vineyards sharing common viticulture practices or environmental conditions, and plastic transcriptome reprogramming was more intense in the year characterized by extreme weather conditions. We also identified a set of genes that lacked plasticity, showing either constitutive expression or similar modulation in all berries. Our data reveal candidate genes potentially responsible for the phenotypic plasticity of grapevine and provide the first step towards the characterization of grapevine transcriptome plasticity under different agricultural systems.
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影响因子：
4.4
作者：
Deluc LG;Grimplet J;Wheatley MD;Tillett RL;Quilici DR;Osborne C;Schooley DA;Schlauch KA;Cushman JC;Cramer GR
通讯作者：
Cramer GR
DOI：
10.1016/s0167-4781(99)00050-0
发表时间：
1999-06-09
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Brosché, M;Strid, Å
通讯作者：
Strid, Å
影响因子：
7.4
作者：
Davies, C;Robinson, SP
通讯作者：
Robinson, SP
影响因子：
4.9
作者：
Debes, Paul V.;Normandeau, Eric;Hutchings, Jeffrey A.
通讯作者：
Hutchings, Jeffrey A.
影响因子：
6.1
作者：
Gatto, Pamela;Vrhovsek, Urska;Velasco, Riccardo
通讯作者：
Velasco, Riccardo