Associations between Season and Gametocyte Dynamics in Chronic Plasmodium falciparum Infections.
Associations between Season and Gametocyte Dynamics in Chronic Plasmodium falciparum Infections.
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DOI：
10.1371/journal.pone.0166699
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Babiker HA
中科院分区：
文献类型：
--
作者：
Gadalla AA;Schneider P;Churcher TS;Nassir E;Abdel-Muhsin AA;Ranford-Cartwright LC;Reece SE;Babiker HA
在疟疾季节性明显的情况下，从无传播的旱季向传播季节的过渡取决于每年雨季开始后蚊子种群的复苏。在传播季节开始时突然爆发疟疾表明，寄生虫在旱季持续存在，并通过增加传播阶段的生产对媒介的重新出现或相关事件作出反应。在这里，我们调查是否恶性疟原虫配子体密度和配子体密度和寄生虫密度之间的相关性显示在苏丹东部的慢性（基本上是无症状）运营商的季节性变化。我们在2001年传播季节招募并治疗了123名疟疾患者。然后，我们在四个不同的连续流行季节每月跟踪他们：传播季节1，无传播季节，临床前期和传播季节2。在从符合当前分析选择标准的25名参与者中收集的样本中，我们分别使用定量PCR（qPCR）和RT-qPCR来定量寄生虫和配子体密度。我们观察到一个显着增加配子体密度和配子体密度和总寄生虫密度之间的显着陡峭的正相关性在临床前期间相比，前无传播季节。然而，总寄生虫或配子体患病率的密度或患病率没有相应增加。在临床前期配子体产量的增加支持了恶性疟原虫可能对环境线索（如蚊子叮咬）作出反应以调节其传播策略的假设。因此，季节性变化可能是引发疟疾不稳定环境中传播的重要因素。
In a markedly seasonal malaria setting, the transition from the transmission-free dry season to the transmission season depends on the resurgence of the mosquito population following the start of annual rains. The sudden onset of malaria outbreaks at the start of the transmission season suggests that parasites persist during the dry season and respond to either the reappearance of vectors, or correlated events, by increasing the production of transmission stages. Here, we investigate whether Plasmodium falciparum gametocyte density and the correlation between gametocyte density and parasite density show seasonal variation in chronic (largely asymptomatic) carriers in eastern Sudan. We recruited and treated 123 malaria patients in the transmission season 2001. We then followed them monthly during four distinct consecutive epidemiological seasons: transmission season 1, transmission-free season, pre-clinical period, and transmission season 2. In samples collected from 25 participants who fulfilled the selection criteria of the current analysis, we used quantitative PCR (qPCR) and RT-qPCR to quantify parasite and gametocyte densities, respectively. We observed a significant increase in gametocyte density and a significantly steeper positive correlation between gametocyte density and total parasite density during the pre-clinical period compared to the preceding transmission-free season. However, there was no corresponding increase in the density or prevalence of total parasites or gametocyte prevalence. The increase in gametocyte production during the pre-clinical period supports the hypothesis that P. falciparum may respond to environmental cues, such as mosquito biting, to modulate its transmission strategy. Thus, seasonal changes may be important to ignite transmission in unstable-malaria settings.
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影响因子：
4
作者：
Churcher, Thomas S.;Blagborough, Andrew M.;Sinden, Robert E.
通讯作者：
Sinden, Robert E.
影响因子：
4.6
作者：
Blanford, J. I.;Blanford, S.;Crane, R. G.;Mann, M. E.;Paaijmans, K. P.;Schreiber, K. V.;Thomas, M. B.
通讯作者：
Thomas, M. B.
影响因子：
4.1
作者：
Cameron A;Reece SE;Drew DR;Haydon DT;Yates AJ
通讯作者：
Yates AJ
影响因子：
2.4
作者：
Buckling, A;Ranford-Cartwright, LC;Read, AF
通讯作者：
Read, AF
DOI：
10.4269/ajtmh.1998.59.582
发表时间：
1998-10-01
影响因子：
3.3
作者：
Babiker, HA;Abdel-Muhsin, ABA;Walliker, D
通讯作者：
Walliker, D