Deep Learning-Based Holographic Polarization Microscopy.
Deep Learning-Based Holographic Polarization Microscopy.
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DOI：
10.1021/acsphotonics.0c01051
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发表时间：
2020-11-18
期刊：
影响因子：
7
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Liu T;de Haan K;Bai B;Rivenson Y;Luo Y;Wang H;Karalli D;Fu H;Zhang Y;FitzGerald J;Ozcan A
偏振光显微镜提供了高对比度的双折射标本，并广泛用作病理学的诊断工具。然而，偏振显微镜系统通常通过分析从不同偏振状态的两个或更多个光路收集的图像来操作，这导致相对复杂的光学设计、高系统成本或需要有经验的技术人员。在这里，我们提出了一种基于深度学习的全息偏振显微镜，该显微镜能够从相位恢复全息图中获得样品的定量双折射延迟和取向信息，同时仅需要向内联无透镜全息成像系统添加一个偏振器/分析器对。使用深度神经网络，可以将来自单一偏振状态的重建全息图像转换为与使用单次计算偏振光显微镜（SCPLM）捕获的图像等效的图像。我们的分析表明，经过训练的深度神经网络可以使用样本特定的形态特征以及全息振幅和相位分布来提取双折射信息。为了证明该方法的有效性，我们通过对各种双折射样品进行成像来测试该方法，所述双折射样品包括例如双折射尿酸盐和曲安奈德晶体。我们的方法实现了类似的结果，SCPLM定性和定量，由于其更简单的光学设计和显着更大的视野，这种方法有可能扩大访问偏振显微镜及其用于医疗诊断资源有限的设置。
Polarized light microscopy provides high contrast to birefringent specimen and is widely used as a diagnostic tool in pathology. However, polarization microscopy systems typically operate by analyzing images collected from two or more light paths in different states of polarization, which lead to relatively complex optical designs, high system costs, or experienced technicians being required. Here, we present a deep learning-based holographic polarization microscope that is capable of obtaining quantitative birefringence retardance and orientation information of specimen from a phase-recovered hologram, while only requiring the addition of one polarizer/analyzer pair to an inline lensfree holographic imaging system. Using a deep neural network, the reconstructed holographic images from a single state of polarization can be transformed into images equivalent to those captured using a single-shot computational polarized light microscope (SCPLM). Our analysis shows that a trained deep neural network can extract the birefringence information using both the sample specific morphological features as well as the holographic amplitude and phase distribution. To demonstrate the efficacy of this method, we tested it by imaging various birefringent samples including, for example, monosodium urate and triamcinolone acetonide crystals. Our method achieves similar results to SCPLM both qualitatively and quantitatively, and due to its simpler optical design and significantly larger field-of-view this method has the potential to expand the access to polarization microscopy and its use for medical diagnosis in resource limited settings.
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影响因子：
3.8
作者：
Greenbaum A;Ozcan A
通讯作者：
Ozcan A
影响因子：
3.8
作者：
Kim, Youngchan;Jeong, Joonwoo;Park, YongKeun
通讯作者：
Park, YongKeun
影响因子：
3.8
作者：
Bishara W;Su TW;Coskun AF;Ozcan A
通讯作者：
Ozcan A
影响因子：
39.2
作者：
MCCARTY, DJ;HOLLANDER, JL
通讯作者：
HOLLANDER, JL
DOI：
10.1111/j.1365-2818.1995.tb03669.x
发表时间：
1995-11-01
期刊：
JOURNAL OF MICROSCOPY-OXFORD
影响因子：
--
作者：
OLDENBOURG, R;MEI, G
通讯作者：
MEI, G