Black dots: High-yield traction force microscopy reveals structural factors contributing to platelet forces.
Black dots: High-yield traction force microscopy reveals structural factors contributing to platelet forces.
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DOI：
10.1016/j.actbio.2021.11.013
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发表时间：
2023-06
影响因子：
9.7
通讯作者：
Sniadecki, Nathan J.
中科院分区：
文献类型：
--
作者：
Beussman, Kevin M.;Mollica, Molly Y.;Leonarda, Andrea;Milesc, Jeffrey;Hocterd, John;Songe, Zizhen;Stollac, Moritz;Hang, Sangyoon J.;Emerya, Ashley;Thomasb, Wendy E.;Sniadecki, Nathan J.
关键词：
测量细胞产生的牵引力可以深入了解它们的行为和生理功能。在这里，我们开发了一种技术（被称为“黑点”），微接触打印荧光微图案到一个灵活的基板上，以测量细胞牵引力，而不限制细胞形状或需要分离的细胞。为了证明我们的技术，我们评估了人类血小板，它可以在人群中产生大范围的力。我们发现施加更大力的血小板具有更大的扩散面积，更圆，并且具有更均匀分布的F-肌动蛋白丝。由于通过这种技术获得的数据产量高，我们能够评估具有交互作用项的多变量混合效应模型，并进行聚类分析以识别数据中的聚类。这些统计技术证明了一个复杂的关系之间的蔓延面积，圆形，F-肌动蛋白分散，和血小板的力量，包括协同效应，显着相关的血小板牵引力。
Measuring the traction forces produced by cells provides insight into their behavior and physiological function. Here, we developed a technique (dubbed ‘black dots’) that microcontact prints a fluorescent micropattern onto a flexible substrate to measure cellular traction forces without constraining cell shape or needing to detach the cells. To demonstrate our technique, we assessed human platelets, which can generate a large range of forces within a population. We find platelets that exert more force have more spread area, are more circular, and have more uniformly distributed F-actin filaments. As a result of the high yield of data obtainable by this technique, we were able to evaluate multivariate mixed effects models with interaction terms and conduct a clustering analysis to identify clusters within our data. These statistical techniques demonstrated a complex relationship between spread area, circularity, F-actin dispersion, and platelet force, including cooperative effects that significantly associate with platelet traction forces.
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影响因子：
3.7
作者：
Palchesko RN;Zhang L;Sun Y;Feinberg AW
通讯作者：
Feinberg AW
影响因子：
9.7
作者：
Polio, Samuel R.;Rothenberg, Katheryn E.;Stamenovic, Dimitrije;Smith, Michael L.
通讯作者：
Smith, Michael L.
影响因子：
64.8
作者：
通讯作者：
--
共聚焦参考游离牵引力显微镜。
影响因子：
16.6
作者：
Bergert, Martin;Lendenmann, Tobias;Zundel, Manuel;Ehret, Alexander E.;Panozzo, Daniele;Richner, Patrizia;Kim, David K.;Kress, Stephan J. P.;Norris, David J.;Sorkine-Hornung, Olga;Mazza, Edoardo;Poulikakos, Dimos;Ferrari, Aldo
通讯作者：
Ferrari, Aldo
影响因子：
3.4
作者：
Feghhi, Shirin;Munday, Adam D.;Sniadecki, Nathan J.
通讯作者：
Sniadecki, Nathan J.