Thermoplastic microfluidic devices and their applications in protein and DNA analysis.
Thermoplastic microfluidic devices and their applications in protein and DNA analysis.
复制标题
DOI：
10.1039/c0an00969e
复制
发表时间：
2011-04-07
期刊：
影响因子：
--
通讯作者：
Fan ZH
中科院分区：
文献类型：
--
作者：
Liu K;Fan ZH
微流体是一种平台技术，已用于基因组学，蛋白质组学，化学合成，环境监测，细胞研究和其他应用。微流控器件的制造材料传统上包括硅和玻璃，但塑料在过去几年中受到越来越多的关注。本文综述了热塑性微流控器件及其在蛋白质和DNA分析中的应用。我们概述了器件的设计和制造方法，然后讨论了表面处理的策略。然后，我们集中在应用热塑性微流体装置的蛋白质分离，免疫测定和DNA分析的几个重大进展。众多的努力之间的比较，以及与检测相关的挑战和创新的讨论，提出。
Microfluidics is a platform technology that has been used for genomics, proteomics, chemical synthesis, environment monitoring, cellular studies, and other applications. The fabrication materials of microfluidic devices have traditionally included silicon and glass, but plastics have gained increasing attention in the past few years. We focus this review on thermoplastic microfluidic devices and their applications in protein and DNA analysis. We outline the device design and fabrication methods, followed by discussion on the strategies of surface treatment. We then concentrate on several significant advancements in applying thermoplastic microfluidic devices to protein separation, immunoassays, and DNA analysis. Comparison among numerous efforts, as well as the discussion on the challenges and innovation associated with detection, is presented.
登录
查看更多内容
影响因子：
7.4
作者：
Bhattacharyya, A;Klapperich, CM
通讯作者：
Klapperich, CM
影响因子：
2.8
作者：
Hupert, Mateusz L.;Guy, W. Jason;Soper, Steven A.
通讯作者：
Soper, Steven A.
影响因子：
2.9
作者：
Gustafsson, Omar;Mogensen, Klaus B.;Kutter, Jorg P.
通讯作者：
Kutter, Jorg P.
影响因子：
3.9
作者：
Dudek, Magdalena M.;Gandhiraman, R. P.;Killard, Anthony J.
通讯作者：
Killard, Anthony J.
影响因子：
7.4
作者：
Emrich, Charles A.;Medintz, Igor L.;Mathies, Richard A.
通讯作者：
Mathies, Richard A.