Lacto-N-tetraose, fucosylation, and secretor status are highly variable in human milk oligosaccharides from women delivering preterm.
Lacto-N-tetraose, fucosylation, and secretor status are highly variable in human milk oligosaccharides from women delivering preterm.
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DOI：
10.1021/pr3004979
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发表时间：
2012-09-07
影响因子：
4.4
通讯作者：
Underwood MA
中科院分区：
文献类型：
--
作者：
De Leoz ML;Gaerlan SC;Strum JS;Dimapasoc LM;Mirmiran M;Tancredi DJ;Smilowitz JT;Kalanetra KM;Mills DA;German JB;Lebrilla CB;Underwood MA
母乳是足月儿的理想营养，但必须补充，才能为大多数早产儿提供足够的生长发育。人乳低聚糖(HMOS)在母乳中含量非常丰富，种类繁多，但对婴儿没有营养价值。HMO似乎至少有两个主要功能：益生活性(刺激肠道内共生细菌的生长)和对病原体的保护。对早产妇女母乳中HMO的调查一直很有限。我们首次使用纳米高效液相芯片/飞行时间质谱仪对15名早产妇女和7名足月分娩妇女的HMOS系列样本中的岩藻糖化和唾液酸化进行了详细的质谱分析。统计分析采用带Levene检验的混合效应模型。我们发现，乳糖-N-四糖，一种核心的HMO，在早产妇女的乳汁中含量更丰富，变异性更高。此外，早产乳中岩藻糖基化的调节不如足月乳中那样好，导致早产与足月分娩的妇女在母亲内部和母亲之间的差异更大。尤其值得临床关注的是，α1，2连接的岩藻糖基低聚糖2‘-岩藻糖基乳糖是一种分泌状态的指标，在几位早产母亲的哺乳期中并不一致。HMO产生的不成熟似乎不会随着哺乳时间的推移而消失，可能与早产儿对坏死性小肠结肠炎、迟发性败血症和相关的神经发育障碍的易感性有关。
Breast milk is the ideal nutrition for term infants but must be supplemented to provide adequate growth for most premature infants. Human milk oligosaccharides (HMOs) are remarkably abundant and diverse in breast milk and yet provide no nutritive value to the infant. HMOs appear to have at least two major functions: prebiotic activity (stimulation of the growth of commensal bacteria in the gut) and protection against pathogens. Investigations of HMOs in milk from women delivering preterm have been limited. We present the first detailed mass spectrometric analysis of the fucosylation and sialylation in HMOs in serial specimens of milk from fifteen women delivering preterm and seven women delivering at term using nano-high performance liquid chromatography chip/time-of-flight mass spectrometry. A mixed-effects model with Levene’s test was used for the statistical analyses. We find that lacto-N-tetraose, a core HMO, is both more abundant and more highly variable in the milk of women delivering preterm. Furthermore, fucosylation in preterm milk is not as well regulated as in term milk, resulting in higher within and between mother variation in women delivering preterm vs. term. Of particular clinical interest, the α1,2-linked fucosylated oligosaccharide 2′-fucosyllactose, an indicator of secretor status, is not consistently present across lactation of several mothers that delivered preterm. The immaturity of HMO production does not appear to resolve over the time of lactation and may have relevance to the susceptibility of premature infants to necrotizing enterocolitis, late onset sepsis, and related neurodevelopmental impairments.
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DOI：
10.1016/0006-291x(67)90404-4
发表时间：
1967-01-01
影响因子：
3.1
作者：
GROLLMAN, EF;GINSBURG, V
通讯作者：
GINSBURG, V
影响因子：
--
作者：
de Leoz ML;An HJ;Kronewitter S;Kim J;Beecroft S;Vinall R;Miyamoto S;de Vere White R;Lam KS;Lebrilla C
通讯作者：
Lebrilla C
影响因子：
8
作者：
Gabrielli, Orazio;Zampini, Lucia;Coppa, Giovanni V.
通讯作者：
Coppa, Giovanni V.
影响因子：
6.1
作者：
Locascio, Riccardo G.;Ninonuevo, Milady R.;German, J. Bruce
通讯作者：
German, J. Bruce
影响因子：
3.9
作者：
Friel, JK;Andrews, WL;Sutradhar, B
通讯作者：
Sutradhar, B