Human coronavirus HKU1 infection of primary human type II alveolar epithelial cells: cytopathic effects and innate immune response.
Human coronavirus HKU1 infection of primary human type II alveolar epithelial cells: cytopathic effects and innate immune response.
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DOI：
10.1371/journal.pone.0070129
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Mason RJ
中科院分区：
文献类型：
--
作者：
Dominguez SR;Travanty EA;Qian Z;Mason RJ
由于原代人呼吸道上皮细胞是呼吸道病原体的天然靶标，因此它们为分离和研究人呼吸道病毒提供了理想的体外系统，这些病毒显示出高度的细胞、组织和宿主特异性。人类冠状病毒HKU 1于2005年首次发现，在全球范围内流行，与儿童和成人的上呼吸道和下呼吸道疾病有关。HCoV-HKU 1的研究一直很困难，因为它不能在连续的细胞系上培养，直到最近才使用原代人纤毛气道上皮细胞从临床标本中分离出来。在这里，我们证明了HCoV-HKU 1可以在空气-液体界面感染并在原代人肺泡II型细胞中连续繁殖。我们不能感染肺泡I型样细胞或肺泡巨噬细胞。HCoV-HKU 1感染的II型肺泡细胞显示形成大的合胞体。在接种后72小时，II型细胞的HCoV-HKU 1感染诱导编码IL 29、CXCL 10、CCL 5和IL-6的mRNA水平增加，而IFNβ水平没有显著增加。这些研究表明，II型细胞是下呼吸道HCoV-HKU 1感染的靶细胞，II型肺泡细胞对感染具有免疫活性，表现出III型干扰素和促炎趋化因子反应，细胞间扩散可能是感染扩散的主要因素。此外，这些研究表明，人肺泡细胞可用于分离和研究引起下呼吸道疾病的新型人呼吸道病毒。
Because they are the natural target for respiratory pathogens, primary human respiratory epithelial cells provide the ideal in vitro system for isolation and study of human respiratory viruses, which display a high degree of cell, tissue, and host specificity. Human coronavirus HKU1, first discovered in 2005, has a worldwide prevalence and is associated with both upper and lower respiratory tract disease in both children and adults. Research on HCoV-HKU1 has been difficult because of its inability to be cultured on continuous cell lines and only recently it was isolated from clinical specimens using primary human, ciliated airway epithelial cells. Here we demonstrate that HCoV-HKU1 can infect and be serially propagated in primary human alveolar type II cells at the air-liquid interface. We were not able to infect alveolar type I-like cells or alveolar macrophages. Type II alveolar cells infected with HCoV-HKU1 demonstrated formation of large syncytium. At 72 hours post inoculation, HCoV-HKU1 infection of type II cells induced increased levels of mRNAs encoding IL29,CXCL10, CCL5, and IL-6 with no significant increases in the levels of IFNβ. These studies demonstrate that type II cells are a target cell for HCoV-HKU1 infection in the lower respiratory tract, that type II alveolar cells are immune-competent in response to infection exhibiting a type III interferon and proinflammatory chemokine response, and that cell to cell spread may be a major factor for spread of infection. Furthermore, these studies demonstrate that human alveolar cells can be used to isolate and study novel human respiratory viruses that cause lower respiratory tract disease.
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影响因子：
3.7
作者：
Mossel, Eric C.;Wang, Jieru;Jeffers, Scott;Edeen, Karen E.;Wang, Shuanglin;Cosgrove, Gregory P.;Funk, C. Joel;Manzer, Rizwan;Miura, Tanya A.;Pearson, Leonard D.;Holmes, Kathryn V.;Mason, Robert J.
通讯作者：
Mason, Robert J.
影响因子：
5.9
作者：
Sattentau, Quentin J.
通讯作者：
Sattentau, Quentin J.
影响因子：
5.4
作者：
de Haan, CAM;Stadler, K;Rottier, PJM
通讯作者：
Rottier, PJM
DOI：
10.1016/s0140-6736(03)12162-9
发表时间：
2003-01-04
期刊：
Lancet (London, England)
影响因子：
--
作者：
Heikkinen T;Järvinen A
通讯作者：
Järvinen A
影响因子：
3.8
作者：
Funk, C. Joel;Wang, Jieru;Mason, Robert J.
通讯作者：
Mason, Robert J.