Prevalence of Helicobacter pylori vacA, cagA, iceA and oipA genotypes in Tunisian patients.
Prevalence of Helicobacter pylori vacA, cagA, iceA and oipA genotypes in Tunisian patients.
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DOI：
10.1186/1476-0711-9-10
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发表时间：
2010-03-19
影响因子：
5.7
通讯作者：
Burucoa C
中科院分区：
文献类型：
--
作者：
Ben Mansour K;Fendri C;Zribi M;Masmoudi A;Labbene M;Fillali A;Ben Mami N;Najjar T;Meherzi A;Sfar T;Burucoa C
幽门螺杆菌的毒力因子主要有：空泡细胞毒素(VacA)、细胞毒素相关基因(CagA)、接触上皮因子抗原(ICEA)基因和外膜蛋白OIPA。在突尼斯，没有关于幽门螺杆菌基因型模式的数据；因此，这项前瞻性的多中心研究是在突尼斯进行的第一项研究，目的是调查突尼斯消化性溃疡、胃癌、MALT淋巴瘤和胃炎患者幽门螺杆菌分离株的VacA、cagA、ICEA和OIPA基因型的患病率。幽门螺杆菌是从281名突尼斯患者的内窥镜活检组织中培养出来的。用聚合酶链式反应检测VacA等位基因、cagA、ICEA和OIPA等位基因。VacA基因s1m1、s1m2和s2m2分别占10.7%、12.5%和45.6%。本研究未检测到s2m1基因。61.6%的分离株携带cagA基因。在60.2%和16%的菌株中分别检出了iceA1和iceA2基因。90.8%的菌株检测到OIPA基因。根据VacA和ICEA基因分型，单个活检标本中存在多株Hp的比例分别为31.4%和23.8%。从胃窦和胃底分离的菌株的比较表明，突尼斯患者感染了两种或两种以上不同cagA、VacA、ICEA和OIPA基因的菌株，不一致的菌株分别为9.6%、4.6%、8.9%和8.5%。结果表明，在281株菌株中，有131株(46%)Hp菌株的毒力与其携带的3~4个毒力因子有关。这些发现在以前的文献中都有描述。突尼斯患者同时被一株或多株幽门螺杆菌定植与同一活检组织中VacA M1/m2和iceA1/iceA2的存在有关。从胃窦和胃底分离的菌株之间的不一致性较高，有利于多定殖化。
Distinct virulence factors of H. pylori have been described: the vaculating cytotoxin (vacA), the cytotoxin associated gene (cagA), the induced by contact with epithelium factor Antigen (iceA gene) and the outer membrane protein oipA. In Tunisia, there are no data regarding the pattern of H. pylori genotypes; therefore, this prospective and multicentre study was the first to be done in Tunisia and aimed to investigate the prevalence of the vacA, cagA, iceA and oipA genotypes of H. pylori isolates from Tunisian patients with peptic ulceration, gastric cancer, MALT lymphoma and gastritis. H. pylori was cultured from endoscopic biopsies obtained from 281 Tunisian patients. The vacA alleles, cagA, iceA and oipA genotypes were determined by PCR. The vacA s1m1, s1m2 and s2m2 were respectively found in 10.7%, 12.5% and 45.6% of strains. The s2m1 genotype was not detected in our study. The cagA was found in 61.6% of isolates. The iceA1 and the iceA2 genotypes were respectively isolated in 60.2% and in 16% of strains. The oipA genotype was detected in 90.8% of strains. Considering the vacA and iceA genotypes, the presence of multiple H. pylori strains in a single biopsy specimen was found respectively in 31.4% and 23.8%. The comparison between strains isolated from antrum and fundus showed that Tunisian patients were infected with two or more strains of different cagA, vacA, iceA and oipA genotypes and the discordance was respectively in 9.6%, 4.6%, 8.9% and 8.5% of strains. Our results showed that in 46% (131 strains among 281), the H. pylori strains were highly virulent in relation of the three or four virulent factors they could carry. These finding were described before in the literature. Tunisian patients were colonized by one or multiple strains of H. pylori in the same time in relation of presence of vacA m1/m2 and iceA1/iceA2 in the same biopsy. The discordance between strains isolated from antrum and fundus was high, and it is in favour of multicolonization.
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影响因子：
1.9
作者：
Go, MF;Cissell, L;Graham, DY
通讯作者：
Graham, DY
影响因子：
9.4
作者：
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2.7
作者：
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影响因子：
3.2
作者：
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影响因子：
4.3
作者：
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