Daily patterns of clock and cognition-related factors are modified in the hippocampus of vitamin A-deficient rats.
Daily patterns of clock and cognition-related factors are modified in the hippocampus of vitamin A-deficient rats.
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在维生素A缺乏大鼠的海马中，时钟和与认知相关的因素的每日模式被修饰。
DOI：
10.1002/hipo.22007
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发表时间：
2012-08
期刊：
影响因子：
3.5
通讯作者：
Anzulovich AC
中科院分区：
文献类型：
--
作者：
Golini RS;Delgado SM;Navigatore Fonzo LS;Ponce IT;Lacoste MG;Anzulovich AC
海马中生物钟和受生物钟控制的认知相关基因的昼夜节律表达对于实现最佳的日常认知表现至关重要。有一些证据表明，类维生素A核受体（RAR和RXR）可以调节不同组织中的昼夜基因表达。在这项研究中，Holtzman雄性大鼠对照组和维生素A缺乏组在整个24小时内处死，海马样品分离每4或5小时。对两组大鼠海马RARα和RXRβ表达水平进行定量，并测定时钟BMAL1、PER1、ROR α和REVERB基因、RORα和REVERB蛋白的每日表达模式，以及认知相关的RC 3和BDNF基因的时间表达。我们的研究结果表明，BMAL1，PER1，RORα和REVERB基因，ROR α和REVERB蛋白的每日变化显着，因此，在大鼠海马中，RC3和BDNF基因的每日振荡表达。维生素A缺乏降低了RXRβ mRNA水平以及PER 1、REVERB基因和REVERB蛋白节律的幅度，并使BMAL 1和RORα mRNA、RORα蛋白、RC 3和BDNF mRNA水平的日峰值发生相移。因此，营养因素，如维生素A及其衍生物类维生素A，可能会调节海马中BDNF和RC 3表达的日常模式，并且它们对于在这个学习和记忆相关的大脑区域中维持分子水平的最佳日常表现至关重要。
The circadian expression of clock and clock-controlled cognition-related genes in the hippocampus would be essential to achieve an optimal daily cognitive performance. There is some evidence that retinoid nuclear receptors (RARs and RXRs) can regulate circadian gene expression in different tissues. In this study, Holtzman male rats from control and vitamin A-deficient groups were sacrificed throughout a 24-h period and hippocampus samples were isolated every 4 or 5 h. RARα and RXRβ expression level was quantified and daily expression patterns of clock BMAL1, PER1, RORα and REVERB genes, RORα and REVERB proteins, as well as temporal expression of cognition-related RC3 and BDNF genes were determined in the hippocampus of the two groups of rats. Our results show significant daily variations of BMAL1, PER1, RORα and REVERB genes, RORα and REVERB proteins and, consequently, daily oscillating expression of RC3 and BDNF genes in the rat hippocampus. Vitamin A deficiency reduced RXRβ mRNA level as well as the amplitude of PER1, REVERB gene and REVERB protein rhythms, and phase-shifted the daily peaks of BMAL1 and RORα mRNA, RORα protein and RC3 and BDNF mRNA levels. Thus, nutritional factors, such as vitamin A and its derivatives the retinoids, might modulate daily patterns of BDNF and RC3 expression in the hippocampus and they could be essential to maintain an optimal daily performance at molecular level in this learning-and-memory-related brain area.
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影响因子：
3.3
作者：
Cocco, S;Diaz, G;Fadda, F
通讯作者：
Fadda, F
影响因子：
56.9
作者：
Balsalobre, A;Brown, SA;Schibler, U
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25
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Storm, Daniel R.
影响因子：
64.8
作者：
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通讯作者：
Reppert, SM
影响因子：
25
作者：
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通讯作者：
Cho, KW