Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain.
Defense against territorial intrusion is associated with DNA methylation changes in the honey bee brain.
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DOI：
10.1186/s12864-018-4594-0
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发表时间：
2018-03-26
期刊：
影响因子：
4.4
通讯作者：
Robinson GE
中科院分区：
文献类型：
--
作者：
Herb BR;Shook MS;Fields CJ;Robinson GE
攻击性受到个体气质差异以及应对逆境时的行为可塑性的影响。DNA甲基化在一段时间内保持稳定，但也可以对特定的环境条件做出反应，因此可能是这两个过程的神经分子调节器。之前的一项研究报告了好斗的非洲蜜蜂和温和的欧洲蜜蜂之间的DNA甲基化差异。我们调查了威胁诱导的攻击是否改变了蜜蜂大脑中的DNA甲基化特征，以响应行为刺激(引发攻击的入侵者蜜蜂或惰性控制)。我们在刺激暴露后5分钟和2小时取样，以检查时间对攻击性表观遗传学特征的影响。攻击性蜜蜂和对照蜜蜂基因组上的单个胞嘧啶-鸟嘌呤二核苷酸(CPGS)存在DNA甲基化差异。刺激后120分钟，攻击性蜜蜂和对照蜜蜂之间有18个单独的CpG位点显示出显著差异。对于CPGS簇，我们报告了4个基因组区域在攻击性蜜蜂和对照蜜蜂之间在5分钟的时间点上差异甲基化，以及50个区域在入侵者暴露后120分钟的时间点上差异甲基化。差异甲基化发生在涉及神经可塑性、染色质重塑和激素信号的基因上。此外，差异甲基化与以前发表的表观遗传差异有显著重叠，这些差异区分了好斗的非洲蜜蜂和温和的欧洲蜜蜂，这表明在进化上保守地使用大脑DNA甲基化来调节攻击性。最后，我们单独确定了具有统计学暗示意义的CPG，这些CPG作为一个群体与攻击行为相关的差异表达基因显著相关，也与参与神经可塑性或神经发育的转录因子的结合位点共同定位。大脑中的DNA甲基化差异与对入侵者的反应有关。这些差异在最初暴露几个小时后增加，并与之前报道的攻击相关基因和神经生物学相关的转录因子结合位点重叠。许多与攻击性实时表达相关的DNA甲基化差异也存在于非洲蜜蜂和欧洲蜜蜂之间，这表明表观遗传调控在攻击行为中具有进化保守的作用。本文的在线版本(10.1186/s12864-0184594-0)包含向授权用户提供的补充材料。
Aggression is influenced by individual variation in temperament as well as behavioral plasticity in response to adversity. DNA methylation is stably maintained over time, but also reversible in response to specific environmental conditions, and may thus be a neuromolecular regulator of both of these processes. A previous study reported DNA methylation differences between aggressive Africanized and gentle European honey bees. We investigated whether threat-induced aggression altered DNA methylation profiles in the honey bee brain in response to a behavioral stimulus (aggression-provoking intruder bee or inert control). We sampled five minutes and two hours after stimulus exposure to examine the effect of time on epigenetic profiles of aggression. There were DNA methylation differences between aggressive and control bees for individual cytosine-guanine dinucleotides (CpGs) across the genome. Eighteen individual CpG sites showed significant difference between aggressive and control bees 120 min post stimulus. For clusters of CpGs, we report four genomic regions differentially methylated between aggressive and control bees at the 5-min time point, and 50 regions differentially methylated at the120-minute time point following intruder exposure. Differential methylation occurred at genes involved in neural plasticity, chromatin remodeling and hormone signaling. Additionally, there was a significant overlap of differential methylation with previously published epigenetic differences that distinguish aggressive Africanized and gentle European honey bees, suggesting an evolutionarily conserved use of brain DNA methylation in the regulation of aggression. Lastly, we identified individually statistically suggestive CpGs that as a group were significantly associated with differentially expressed genes underlying aggressive behavior and also co-localize with binding sites of transcription factors involved in neuroplasticity or neurodevelopment. There were DNA methylation differences in the brain associated with response to an intruder. These differences increased in number a few hours after the initial exposure and overlap with previously reported aggression-associated genes and neurobiologically relevant transcription factor binding sites. Many DNA methylation differences that occurred in association with the expression of aggression in real time also exist between Africanized bees and European bees, suggesting an evolutionarily conserved role for epigenetic regulation in aggressive behavior. The online version of this article (10.1186/s12864-018-4594-0) contains supplementary material, which is available to authorized users.
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DOI：
10.1177/1073858415579635
发表时间：
2015-10
期刊：
The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry
影响因子：
--
作者：
Heyward FD;Sweatt JD
通讯作者：
Sweatt JD
DOI：
10.1073/pnas.1412306111
发表时间：
2014-08-26
影响因子：
11.1
作者：
Li-Byarlay, Hongmei;Rittschof, Clare C.;Robinson, Gene E.
通讯作者：
Robinson, Gene E.
影响因子：
3.6
作者：
Gold KS;Brand AH
通讯作者：
Brand AH
影响因子：
25
作者：
Elliott, Evan;Ezra-Nevo, Gili;Chen, Alon
通讯作者：
Chen, Alon
影响因子：
14.9
作者：
Ji H;Vokes SA;Wong WH
通讯作者：
Wong WH