Contrasting transcriptional responses to Fusarium virguliforme colonization in symptomatic and asymptomatic hosts.
Contrasting transcriptional responses to Fusarium virguliforme colonization in symptomatic and asymptomatic hosts.
复制标题
有症状和无症状寄主对镰刀菌定植的转录反应的对比。
DOI：
10.1093/plcell/koaa021
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发表时间：
2021-04-17
期刊：
影响因子：
--
通讯作者：
Day B
中科院分区：
文献类型：
--
作者：
Baetsen-Young A;Chen H;Shiu SH;Day B
镰刀菌的广泛寄主范围代表了一个独特的比较系统，可以识别和定义无症状的单子叶寄主玉米（Zea mays）和有症状的单子叶寄主大豆（Glycine max）之间的差异诱导反应。利用一种基于时间的、比较转录组的方法，我们观察到受感染玉米根组织的早期基因表达谱表明，病原体耐受性与衰老抑制转录调控因子的快速诱导一致，包括ANACs（拟南芥NAM/ATAF/CUC蛋白）和乙烯响应因子。相比之下，大豆衰老相关过程的表达与疾病症状发展的出现是一致的，这表明病原诱导的衰老是大豆病原易感性的关键途径。基于本文所述的分析，我们假设根系衰老是有症状与无症状宿主-真菌相互作用中定植和疾病进展的主要因素。这一过程也支持F. virguliforme在生物营养到坏死转化过程中的生活方式和毒力。进一步支持这一假设的是全面的共表达和比较转录组分析，总的来说，支持了坏死细胞活化衰老的新概念。我们认为，F. virguliforme在有症状的宿主体内创造了一个环境，通过转录组重编程有利于易感性，并如本文所述，在真菌发育的坏死阶段诱导与衰老相关的途径。镰刀菌诱导的根系衰老在有症状寄主（如大豆）和无症状寄主（如玉米）中受到不同的调控。
The broad host range of Fusarium virguliforme represents a unique comparative system to identify and define differentially induced responses between an asymptomatic monocot host, maize (Zea mays), and a symptomatic eudicot host, soybean (Glycine max). Using a temporal, comparative transcriptome-based approach, we observed that early gene expression profiles of root tissue from infected maize suggest that pathogen tolerance coincides with the rapid induction of senescence dampening transcriptional regulators, including ANACs (Arabidopsis thaliana NAM/ATAF/CUC protein) and Ethylene-Responsive Factors. In contrast, the expression of senescence-associated processes in soybean was coincident with the appearance of disease symptom development, suggesting pathogen-induced senescence as a key pathway driving pathogen susceptibility in soybean. Based on the analyses described herein, we posit that root senescence is a primary contributing factor underlying colonization and disease progression in symptomatic versus asymptomatic host–fungal interactions. This process also supports the lifestyle and virulence of F. virguliforme during biotrophy to necrotrophy transitions. Further support for this hypothesis lies in comprehensive co-expression and comparative transcriptome analyses, and in total, supports the emerging concept of necrotrophy-activated senescence. We propose that F. virguliforme conditions an environment within symptomatic hosts, which favors susceptibility through transcriptomic reprogramming, and as described herein, the induction of pathways associated with senescence during the necrotrophic stage of fungal development. Fusarium-induced root senescence is differentially regulated in symptomatic hosts (e.g. soybean) versus asymptomatic hosts (e.g. maize).
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
4.6
作者：
Chowdhury, Supriyo;Basu, Arpita;Kundu, Surekha
通讯作者：
Kundu, Surekha
影响因子：
11.6
作者：
Baetsen-Young, Amy;Wai, Ching Man;Day, Brad
通讯作者：
Day, Brad
影响因子：
5.6
作者：
Chen Y;Yin H;Gao M;Zhu H;Zhang Q;Wang Y
通讯作者：
Wang Y
影响因子：
9.4
作者：
Deveau, A.;Kohler, A.;Martin, F.
通讯作者：
Martin, F.