Immunohistochemical characterization and functional identification of mammary gland telocytes in the self-assembly of reconstituted breast cancer tissue in vitro.
Immunohistochemical characterization and functional identification of mammary gland telocytes in the self-assembly of reconstituted breast cancer tissue in vitro.
复制标题
乳腺远程细胞在体外重建乳腺癌组织自组装中的免疫组织化学表征和功能鉴定。
DOI：
10.1111/j.1582-4934.2012.01646.x
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发表时间：
2013-01
影响因子：
5.3
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Mou Y;Wang Y;Li J;Lü S;Duan C;Du Z;Yang G;Chen W;Zhao S;Zhou J;Wang C
端粒细胞作为一种特殊的基质细胞存在于乳腺组织中，在乳腺上皮-间质的平衡中可能起着重要的作用。考虑到不同类型的乳腺间质细胞影响乳腺癌的发生和发展，TCS在这一过程中可能有其独特的作用。我们在这里研究了TCS在重组乳腺癌组织自组装中的作用。我们将原代分离的TCS和其他乳腺基质细胞与乳腺癌EMT-6细胞在胶原/Matrigel支架中共同培养，以在体外重建乳腺癌组织。应用组织学方法，我们研究了重组乳腺癌组织中TCS的免疫组织化学特征和潜在功能。原代乳腺基质细胞胞浆突起细长重叠，重建的乳腺癌组织表达c-kit/CD117、CD34和波形蛋白。透射电子显微镜显示，端粒细胞样细胞与乳腺癌细胞以及其他间质细胞紧密联系，可能是通过膜对膜接触直接连接相邻细胞的桥梁。与EMT-6单独的癌组织片相比，增殖指数分析和TUNEL检测显示，TCS和其他乳腺基质细胞促进了典型的巢状结构的形成，促进了乳腺癌细胞的增殖，并抑制了细胞的凋亡。综上所述，我们成功地在体外重组了乳腺癌组织，TCS在EMT-6/基质细胞重组乳腺癌组织中具有潜在的自组装功能。
Telocyte (TC) as a special stromal cell exists in mammary gland and might play an important role in the balance of epithelium-stroma of mammary gland. Considering that different types of breast interstitial cells influence the development and progression of breast cancer, TCs may have its distinct role in this process. We here studied the roles of TCs in the self-assembly of reconstituted breast cancer tissue. We co-cultured primary isolated TCs and other breast stromal cells with breast cancer EMT-6 cells in collagen/Matrigel scaffolds to reconstitute breast cancer tissue in vitro. Using histology methods, we investigated the immunohistochemical characteristics and potential functions of TCs in reconstituted breast cancer tissue. TCs in primary mammary gland stromal cells with long and thin overlapping cytoplasmic processes, expressed c-kit/CD117, CD34 and vimentin in reconstitute breast cancer tissue. The transmission electron microscopy showed that the telocyte-like cells closely communicated with breast cancer cells as well as other stromal cells, and might serve as a bridge that directly linked the adjacent cells through membrane-to-membrane contact. Compared with cancer tissue sheets of EMT-6 alone, PCNA proliferation index analysis and TUNEL assay showed that TCs and other breast stromal cells facilitated the formation of typical nest structure, promoted the proliferation of breast cancer cells, and inhibited their apoptosis. In conclusion, we successfully reconstituted breast cancer tissue in vitro, and it seems to be attractive that TCs had potential functions in self-assembly of EMT-6/stromal cells reconstituted breast cancer tissue.
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特细胞是 PEComas 和 GIST 的共同起源细胞：这是一个有证据支持的假设。
影响因子：
5.3
作者：
Ardeleanu C;Bussolati G
通讯作者：
Bussolati G
影响因子：
5.3
作者：
Ceafalan L;Gherghiceanu M;Popescu LM;Simionescu O
通讯作者：
Simionescu O
影响因子：
5.3
作者：
Manole CG;Cismaşiu V;Gherghiceanu M;Popescu LM
通讯作者：
Popescu LM
DOI：
10.1073/pnas.0808932106
发表时间：
2009-01-13
影响因子：
11.1
作者：
Fischbach, Claudia;Kong, Hyun Joon;Mooney, David J.
通讯作者：
Mooney, David J.
影响因子：
3.2
作者：
Hinescu, Mihail E.;Ardeleanu, Carmen;Popescu, Laurentziu M.
通讯作者：
Popescu, Laurentziu M.